...site-specifically label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, ...the attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop...Xenopus oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that...

...recognition, and DNA cleavage. RNA loading Once supplied with a guide RNA — whether it’s an sgRNA or a ...lab mutated the residues in this loop to produce SuperFi-Cas9, a high-fidelity variant that still retains... University of California, San Francisco, with support from National Institutes of Health R01-GM129325...

...language or another. Supported organisms: This section lists the genomes supported by each tool. The genomes...insights are hard to appreciate without computational support. Indeed, rational sgRNA design is not possible ...

...New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the...techniques. It’s also great for teachers looking to supplement their curriculum. From the details about personal...

... a lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament...

...India, and Brazil.  We've also created a robust support system for scientists using materials in our repository...repository, from our customer and science support to our efficient and easy tech transfer process to our...

...public support for research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science...

... endA Delta(sr1-recA) mcrB mrr hsdS20 (rB- mB-) supE44 ara14 galK2 lacY1 proA2 rpsL20(StrR) xyl5 lambda...lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega ...McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Delta(lac- proAB) [F traDelta36 proAB lacIqZDeltaM15...Invitrogen F– mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ...

...of the founding groups of the field under the supervision of Prof Jim Collins and specialising in engineering...biotechnology and has been directly involved in supervising many successful iGEM undergraduate projects. ...

...~10 years! We're extremely proud to be able to support scientists by bringing you all the plasmids you...storing them, providing quality control and customer support, making plasmids easy for you to find and request...

..., we must be making a dent in the global coffee supply… My back-of-the-napkin calculations put the daily...community. It has been great to hear all the words of support from scientists using Addgene in their labs. And...

...smooth and simple as possible. We are the Customer Support Specialists here at Addgene. If you’ve called our... store your plasmids or viral items, technical support, and more. ...

...this post for a few reasons: 1) I think phage are super cool and have considered getting a tattoo of my ...would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids on this...

..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played...

...by our depositors, used by our requestors, and supported by our repository. You can enjoy these stories.... In 2024, we will be actively seeking ways to support a global community where every researcher feels...

...Addgene's Europe office no longer handles customer support inquiries. The office is now focused on scientific... first to be located in London and focused on supporting European scientists. Born a french frog, I moved...

...this photo!  Here, I was also given a mixture of support and independence that allowed me to succeed. As...marketing and content teams for their help and support throughout the past six months!...

...fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in...GFP aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1...

...your data to a repository (Figshare is currently supported by ReFigure beta) you can include it in a ReFigure... ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering this...

...hands-on science lab kits and digital curricula to support them. Caitlin’s take on her job as an SME: “I write...a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs],” says Caitlin...