Choosing the B(right)est Fluorescent Protein: Photostability
Type
Blog Post
...a fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend... 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on power is...to select a photostable fluorescent protein.
Photobleaching is the irreversible destruction of a fluorophore...influence of light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable...fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may display reversible...destroyed.
Factors that affect photostability
The photobleaching rate of a fluorophore primarily depends on ...excitation wavelength. A fluorophore is less likely to bleach when it is excited off the peak wavelength, since...