...was particularly helpful for securing funding to support this effort. The future of reproducibility workshops...Science mini-grant, which will fund a project to support a dedicated website to host all the materials in...collaboration is that it is driven by academics, with support of industry partners, not the other way around....

...Nobel Prize in Chemistry for his contributions to super-resolution light microscopy, comes from his recent...Communications article “SRpHi ratiometric pH biosensors for super-resolution microscopy.” Endosomes are historically...studying endosomes, the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet...

...genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional 6 gRNAs...expression of the MutL mutant enables transient suppression of DNA repair during oligonucleotide integration...Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher...connectors” that link tags to the targeted gene are supplied to the reaction from a prebuilt donor plasmid ...sequences for homology arms and the guide RNA are supplied as annealed synthetic oligos - eliminating the...

...Commun. 2016 Bo Huang spGFP Split superpositive GFP Split-superpositive GFP reassembly is a fast, efficient...Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP, our most-requested GFP(1-10) and GFP...

...of xanthine into guanine, and without xanthine supplied in the media, these bacteria are unable to divide...was able to grow this strain in minimal media supplemented with various caffeinated beverages, but not ...Angela Holmes Jin Zhang’s lab is developing new super-resolution techniques for live-cell imaging of the...

...enhancers and chromatin looping play important supporting roles. Enhancers are cis regulatory DNA sequences...promoters or genes each enhancer is interacting with. Super enhancers are particularly good models for building...simultaneously characterize the ‘social networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin...

...the pAdEasy-2 packaging plasmid; Supplies viral protein E4 E1 Supplied in trans by 293 or 911 packaging...adenovirus has two genes deleted: E1 and E3. E1 is supplied by the adenovirus packaging lines 293 or 911; ...One can also co-transform standard BJ5183 with supercoiled pAdEasy™ and the shuttle vector, but this method...capacity E4 Essential for viral transcription; Supplied by the pAdEasy-1 packaging plasmid or 911E4 packaging...

...mammalian cells) to collect the cell culture supernatant for further processing. It may be helpful to ...immobilized ligands are incubated with cell culture supernatant containing antibodies. The antibodies bind the...Rockberg J, Chotteau V. Small-scale bioreactor supports high density HEK293 cell perfusion culture for...

... Association (PTA) has been instrumental in supplementing that curriculum. It’s through the PTA that I...Spangler Science The Fab Lab Middle school: supplementing the science curriculum Sharing my expertise ...you want to make sure their STEM education is supplemented. A 2009 study by the NSF showed that among US...

...binding) Google Maps: Enhancers and genome math Suppose that you wish to use Google maps to find all coffee...disrupt these enhancers. A real-world experiment Suppose you run a ChIP-seq experiment (think “GPS for proteins...we all know and love. To understand compaction, suppose you have a piece string that’s 10 meters long. ...

...but you have definitely reaped the benefits. We support everyone that works with Addgene in some way. For...the development process at Addgene. Diversity supports our innovation Our software development team is...This can lead to confusion as to what the code is supposed to be doing. When this happens, we discuss and...

...Customizable to our specific needs Modern and well-supported, so we could focus on our development instead ...encouraged to see that a community of Nextflow supporters existed who had created a shared resource of ...modern architecture of Nextflow included built-in support for containers, such as Docker. If you’ve heard...

...successful mentor relationships, asking for mentor support, and choosing your mentor. What isn’t discussed...mentor learns where you are at and the level of support you need from them, and you can understand the ...Be My Mentor? Finding and Asking for Mentoring Support   ...

...of the high cell-density they support; however, minimal media supplemented with M9 salts may be preferable...measure of control such as the pLys plasmid, which suppresses basal T7 expression. The pLys plasmid contains...

... is customer support, but there are eight other teams who still retain a customer support element amongst...responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day operations...

...FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, which...additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It is important to consider...transiently transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence...

...perturbed by the experiment The loading control is supposed to control for, well, loading errors. When using...A. V., “Stain-Free total protein staining is a superior loading control to β-actin for Western blots,”...(2013). Stain-Free total protein staining is a superior loading control to β-actin for Western blots. ...

... endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008). They have...into the endogenous allele of the STAG2 tumor suppressor in human cells (Kim et al, 2016). The Waldman...components as ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery...

...play. Photoswitches can be used to activate or suppress secondary signaling pathways with light. This ... closely read the publication, including the supplemental data. You’re most likely to find thorough characterization...spectral and kinetic activation information, in the supplemental data instead of in the main text.  Then, check...

...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...Enterprise crew fight against a genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie...The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous wolf...