...plasmid. Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors...vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure 3: Comparison...genomic modification. In this system, Cas9 must be supplied on a separate plasmid. Multiplexing in Drosophila...from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid. Multiplexing in E. coli...

... NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply concerned...with partners across the research ecosystem to support the essential work of our scientific community ...funding changes are impacting your work and what support would be most valuable from Addgene during this...

...and professional experience are prized skills. Supervise undergrads or less experienced grads Manage a ...something big like plan a conference Recruit many supporters and mentors that will speak for you (and you ...get real feedback Write whenever you can, find supportive editors so your first drafts are always excellent...

...planning meetings with each trainee to create a supportive, growth-oriented lab culture. The aim of these...summarized briefly below (and available of in the supplement of this paper), help each person organize his...Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists: Seeking Feedback Management...

...metastasis CRISPR screen. The Chen Mouse Tumor Suppressor Gene CRISPR Knockout Library is an AAV CRISPR...CRISPR knockout library targeting mouse tumor suppressor genes (TSG). Find the Mouse Metastasis CRISPR-Cpf1...Double Knockout Library Find the Mouse Tumor Suppressor Gene CRISPR Knockout Library If you have a new...

... is resistance to change, public support is weak, government support is “feckless”.  So what can be done...from Harvard Medical School and a long list of supporters from all of the research centers in Boston.  ...

...results section, but you had to dig through the supplemental files to see the actual data. Now you’re wondering...datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it possible for...sgRNA-level sequence read scores. The original supplemental data tables available with the publication are...

... high school friends. In my teenage years I was super shy - there was even a time when I was too nervous...writing scientific manuscripts and, as one of my supervisors warned me, the swift gratification of publishing...roles can include positions, like mine, that help support academia but the skills researchers gain doing ...

...research area is not as important as choosing a supportive, positive role model. By contrast, as a postdoc...is crucial that you choose an advisor that will support your growth in your science career. Will you be...thinking about a non-academic path, will the PI support this path and help you pursue any career alternative...

...during the pandemic and in the future, depends on support from the scientific community. Deposits of new ... be deposited into repositories, and financial support to maintain repositories’ capacity for storing,...all ways in which the scientific community can support repositories. Repositories are essential for the...

...Candidate, University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University ...Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

... 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of gene editing...Kim et al 2014) have shown that this technique supports extremely high frequencies of genome cutting and...nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...

...Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial growth, and is not...like gentamicin, neomycin is typically used to suppress bacterial growth. The selection agent should not...

...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...Fishell   Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...

...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers... a luciferase substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite the...

...COVID-19 pandemic, students will face a reduced supply of internships as a result of the necessary restrictions...the request to help your recommenders deliver a superb and detailed portrayal of yourself. Be mindful ...likelier you will end up with a stellar letter of support.        5. Application submission  Set a calendar...

...adenovirus genes – E4, E2a, and VA – which are supplied on a helper plasmid and mediate efficient viral...including the ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to...making it replication deficient. This is typically supplied by the production cell line (HEK-293) during production...

...openUC2, which I am a co-founder of), that aim to support the community in developing better, more sustainable...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...-based models and the use of taxpayer money to support this research. But there is a move towards more...

...aspects of my work but also found fulfilment in supporting my team’s development and career growth. This...check-ins, which helped them stay on track and feel supported. Another team member was highly self-motivated... served as a great "baby project," offering a supportive environment to build my management skills. Finally...

...save yourself a headache later on. Enhancer and suppressor screens These types of screens are very similar...identify manipulations which either enhance or suppress the previously defined disease phenotype. These... a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections differ from screens...