...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  ...either.  Test tubes aren’t cheap everywhere Lab supplies and equipment are more precious in less resourced...Labs does an amazing job shuttling unwanted lab supplies to places that really need them.  When I was in...stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy to...

...and have always been, a community resource that supports both toolmakers and tool users. We encourage you...Educational Resources As part of our commitment to supporting your research, Addgene is creating an array of...Through our incredible community, including your support, we have enabled scientists to easily share plasmids...Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community for decades...Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative grant (U24NS119916 )...

...PA-FPs have helped to revolutionize the field of super resolution microscopy. PA-FPs, especially the monomeric...monomeric, reversible type, are very well suited for super resolution imaging, where the power of the technique... reconstruct an image. The best PA-FPs used for super-resolution imaging usually have the following characteristics-high...fluorescence wavelengths. Indeed, within the world of super resolution microscopy, PA-FPs have helped create...are vastly improved over the conventional FPs for super-resolution imaging, they still do not emit the large...being engineered. By combining PA-FPS with superresolution microscopy, scientists now have at their fingertips...

...classes and participating in these is a great way to support your favorite local exercise spot or find something...PhysicalDistancing. Social interactions and #social support are even more necessary during these times of stress... stress due to #COVIDー19. Receive and provide support while being safe. @GHP_HarvardChan — Shekhar Saxena...testing for COVID-19. You can also give back by supporting local businesses (ex: purchasing a gift card .... Think about reproducibility best practices Support your future experiments and other researchers by...

... inducing immunosuppressive measures, for example the upregulation of the immunosuppressive cell surface...factors for chronic inflammation which in turn suppresses cancer immunosurveillance. The presented study...surface receptor PD-1. This immunosuppressive environment in turn may represent a favorable state for tumor...Future treatments may need to target this immunosuppressive reaction in order to improve therapeutic outcomes...

...You need the support of your institute to do this one. Universities will usually only support tenure track...This criterion is best proven through letters of support from experts in the field. You must also be able...Performing peer review at the request of your supervisor is not considered peer review. The peer review...very attractive option.    Your Expert Letters of support can mean that your case gets approved, denied or...Request for Further Evidence (RFE). Letters of support are the only opportunity that you are given to ...

...particular the roles of proto-oncogenes, tumor suppressors, and their protein products. Proto-oncogenes ...survival, growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from...compare the roles of proto-oncogenes and tumor suppressors to the accelerator and brake pedal of a vehicle...down. Similarly, a mutation that turns a tumor suppressor “off” is analogous to a brake pedal that does...specific genes involved. Downregulation of tumor suppressors and upregulation of proto-oncogenes ­­­can directly...

...your media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...culture media Changing to a richer media or adding supplements can improve your cell density, leading to an ...normally recommended antibiotic concentration. Add supplements to the media You can achieve higher cell density...

...features previously only visible via techniques like super-resolution microscopy or electron microscopy. Since... of specific targets you can label in a sample. Super-resolution microscopy (SRM), a family of imaging...targets. What are some of the limitations?  A “super-resolution” technique that uses common reagents,....73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical Synapses in the Brain. ...Diederich B, Heintzmann R, Schermelleh L (2022) Super-resolution microscopy: a brief history and new avenues...

...efficiency supE44 (glnV44)   Suppression of the amber (UAG) stop codon by glutamine insertion supF (tyrT)...tyrT)   Suppression of the amber (UAG) stop codon by tyrosine insertion λ-thi-1 or thi1 Mutation in thiamine...(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (...

... be your actual mentor? Is there a chance the supervisor will be moving on soon?   Will you get to meet...interviewing: Lab members speak disrespectfully of supervisors and/or each other. You are not allowed to speak... before answering questions about lab culture. Support for diverse career paths I hope by now you are ...making machine. Choose one that is a true mentor, supporting career development and growth. Have lab alumni...someone(s) train you technically? Will the PI/supervisor look at your data and suggest follow up experiments...

...corresponds to the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid ...sample. In these instances, please note that the supercoiled dimer band can sometimes overlap with the band...development to examine plasmid topologies (e.g. supercoiled, open circular, linearized, etc.) (Schleef et...Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells. J...

... and logistic support to increase the chances of success, but also nurture a supportive and protective...mentee, where (s)he feels truly understood and supported, and can more fully identify with and be inspired... Vela, who reiterated the Spanish government’s support for the IMP program as well as for the Spanish ...public and private institutions also showed their support and hope for the IMP program. The conference also...

...particles into the culture supernatant and you can generally harvest the supernatant containing viruses 24h...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target ... process. You have to centrifuge the culture supernatant in order to precipitate the viral particles. ...Some protocols suggest adding sucrose to the supernatant to create a gradient of density that favours ...

...performance and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...SNAP-tag and HaloTag ligands are available from suppliers like New England Biolabs and Promega (though if.... If you want to use far-red rhodamine dyes for super-resolution imaging, HaloTag gives better performance...Toomre, D. (2019). Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags...

...programs are still doing an inadequate job of supporting science trainee transition into diverse, successful...amplified by systemic biases which, in the absence of support and structure, prevent science from attracting ... of Massachusetts Medical School. It has been supported by a Steering Committee and working groups with...Institute of General Medical Sciences (NIGMS) will support the development and implementation of collections...

...available in both trial- and full-size, with assay-supported recommended uses and education content. This work...our commitment to top notch quality service and support.  Alongside our material offerings, in 2022 we...expanding our suite of materials and services to support more research and innovation around the world. ...will remain the same is Addgene’s commitment to supporting the research community through sharing science...

...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...industry concerns.” 5)      In the aftermath of the Supreme Court’s decision to ban gene patents in a case ...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...

...Behind-the-scenes support from their bosses and employers. Availability of superiors for consultation,...self-directed, but need precise leadership and support from their superiors (beware of not micromanaging the scientist...

...from the American Society of Plant Biologists and support from the National Science Foundation, ABRC’s outreach...provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional videos and datasheets...classroom is that staff from the ABRC are here to support you! From questions about growing conditions, to...e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach today Over the...