...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology...

...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but...

...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into...

...Structural Genomics Consortium (SGC) Plasmids Opher Gileadi Lab plasmids Haian Fu Lab plasmids The TREAT-AD...AD and creating high-quality, well-characterized lead molecules for prioritized targets that are valued...

... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions...

...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ...

...plasmids required to practice CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in zebrafish. The Joung Lab...expression of a transcript encoding the Cas9 endonuclease. Details for producing mRNA suitable for expression...

...plasmids required to practice CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in human cells. The Cas9 expression...expression of a transcript encoding the Cas9 endonuclease. Both vectors also express a C-terminal 3X FLAG...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ...fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent ...

...well-characterized antibodies in an open science model. Learn more about IPI (Link opens in a new window) Glypican...DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody Collection...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does...

...pSB700 64046 Mammalian/Lentiviral none S. pyogenes Cerulean Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian...multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate...plasmid for the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid...

...Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing...systems for novel functions. Addgene is working with leading synthetic biologists around the world to facilitate...

...Addgene as well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody ...popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their applications in ...

...gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids ...

...of host cellular machineries, requiring only a nuclease-deficient Cas9 (dCas9) protein and a customized.... , Jiang et al. , Hwang et al. ), is that the nuclease-deficient mutant could silence transcription on...

...rapid induction with efficient tetracycline uptake. Please search our full site for all tetracycline plasmids...Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (MAT2A) which...