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  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...-to-day work. Indeed, viral vector production facilities frequently use qPCR with a standard curve to ...

  3. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...flanked by HIV-1 long terminal repeats (LTRs) that facilitate viral packaging and host genome integration. ...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific...tolerance, and optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis (Lui et al., 2012, Ni et al., 2008, ...Zhu L, Wang H (2012) Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility. PLoS ...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    .... In vivo, these recombination reactions are facilitated by the recombination of attachment sites from...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry vector....

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...screens Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of...-024-07259-6. PMID: 38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...’ resistance to beta-lactam antibiotics, like penicillin. These specific drugs attack the cell wall of... of survival. This was the whole basis of how penicillin was discovered! Of course we wouldn’t have such...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    ...Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic cells...

  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... Rab35 to understand their roles in forming the cilia that help establish left-right organization of the...2023). Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer ...

  14. Protocol - Bacterial Transformation

    Type
    Protocol
    ...containing agar plate. This step is not critical for Ampicillin resistance but is much more important for other...heat-shock Shorten or skip the outgrowth (for Ampicillin resistance it is ok to completely skip the outgrowth...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... of other organisms, such as sphingomonads and bacillus.  ADH1 The ADH1 negative repressible promoter ...synthetic cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...polymerase prior to fragmentation can greatly facilitate downstream manipulations. Phi29 polymerase is...your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5 min at -20°...

  17. Plasmids for Stem Cell Research

    Type
    Collection
    ...BCL2L1, and EBNA1 from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem...
Showing: 81 - 100 of 253 results