...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ...

...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...

... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...

...03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

... interview. Each interview is an opportunity to learn something and then apply it to the next interview...

...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...genome editing. For more information on AAV-CRISPR, please see this blog post. Browse the article from Ran...particles produced during a lytic cycle are quickly released through cell lysis. Episomal stability enables...

...the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find...

...compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells...

...science happens in a vacuum!  Munro and Pelham learned of these new antibodies and identified Myc1-9E10...