....    Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources...

...and easy ways to share your documents with your colleagues. Start small by identifying the protocols, guides...

...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors...

...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...to observe what’s happening inside cells for at least a couple of decades. Green is the classic color,...

...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently...DREADDs, their ligands, and signaling properties. Please see the text or table 1 for details. DREADD Ligands...for a discussion of alternative DREADD ligands, please see the text Browse Addgene's collection of DREADD...based LGICs, their effect, and outcome in neurons. Please see the text or table 2 for details. Table 2. Descriptions...

...sequence with one or more enzymes.   Click here to learn how to run an agarose gel     We’ve worked with...

...CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there are many other Cas proteins as well...

... an opportunity for the researchers involved to learn techniques from each other, access specialized equipment...

... topic suggestions for our Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick...

...Resources on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR ...

... within the past six weeks. Through Addgene, I learned the basics of lab safety, general work skills —...

... to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral service Find optogenetics...

...nervous (I mean I WAS presenting to three department leads, I probably should have been nervous), but I wasn...

...variants of unknown significance – mutations with unclear effects on disease pathology - to determine if ...

...29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase ...

...communicating your research by guest blogging here, just leave a comment if you're interested and our team will...

...description of 2nd and 3rd generation lentiviruses, please review 2nd vs 3rd generation above . Briefly, 2nd...Ther. 2005. 5(4): 387-398. PubMed . HIV-1 Genome Nuclear Import Is Mediated by a Central DNA Flap. Zennou...and partially spliced transcripts to facilitate nuclear export. Tat in trans Second generation only; Trans-activator...stimulates the expression of transgenes via increased nuclear export. LTR Sub-components: U3 R Sub-element: TAR...

...I knew anything about being an editor - that I learned on the job. The day-to-day work of a science editor...

...genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of your choice...