...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

...VchINT does not recruit other nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms...

...overexpression of fusion proteins it requires can lead to aberrant subcellular localization of the studied... and neurodegeneration. He now spends his time learning and writing about science whenever he's not off...

...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...

...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently...DREADDs, their ligands, and signaling properties. Please see the text or table 1 for details. DREADD Ligands...for a discussion of alternative DREADD ligands, please see the text Browse Addgene's collection of DREADD...based LGICs, their effect, and outcome in neurons. Please see the text or table 2 for details. Table 2. Descriptions...

... viral vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...Resources on Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols...

...containing glucose and L-arabinose at 37oC for at least 12 hours, or at lower temperatures for longer. Cultures... induction of expression… but longer expression leads to greater amount of gas vesicles and increased ...

...easy to export data from the ELN directly into at least one and preferably more than one of these five general...other side of the pond through interacting with colleagues in Edinburgh. You can follow Rory on Twitter ...

...base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): 420. PubMed PMID: 27096365...editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed PMID: 29160308...

...only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for. The result is redundancy...organism. So what are the evolutionary constraints that lead to these preferences and what can we do about them...

...ticker tape By Brian O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional...Application section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts...

...optimization within your experiment. Read on to learn more about the various controls that can be used...biological controls is independence: at the very least you should repeat your whole experiment from start...

...description of 2nd and 3rd generation lentiviruses, please review 2nd vs 3rd generation above . Briefly, 2nd...Ther. 2005. 5(4): 387-398. PubMed . HIV-1 Genome Nuclear Import Is Mediated by a Central DNA Flap. Zennou...and partially spliced transcripts to facilitate nuclear export. Tat in trans Second generation only; Trans-activator...stimulates the expression of transgenes via increased nuclear export. LTR Sub-components: U3 R Sub-element: TAR...

...governments exist in reality and science is how we learn about reality. Formally, a government can be defined...public discourse, many scientists have chosen to leave the lab and march. Unintended consequences While...

...tip: When designing your PCR, ensure there is at least ~200 base pairs of sequence flanking the editing...find a null clone, you should screen, at the very least, 4 clones. Did you use a dual guide system to generate...

... 1). In general, the monomeric state of a probe leads to less interference with the oligomeric state of...indicators NIR-GECOs were developed based on mIFP6. Learn more about the characteristics, performance, and...

...loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the genome that...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...

... in the classroom on our blog. Now that you’ve learned more about popular model organisms in the lab, ... about plasmids in our Plasmids 101 blog series Learn more about CRISPR and genome editing in our CRISPR...