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  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...temperature). Photostability: Fluorescent molecules gets bleached (i.e. lose the ability to emit light) after prolonged...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

  3. MXS Chaining

    Type
    Blog Post
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids ...

  4. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...target the CNS or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they... Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene expression...

  5. AAVs in Retinal Gene Therapy

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    Blog Post
    ...AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression in...development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push ...

  8. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...called a standard. The ideal standard curve has at least five dilutions, with each step of the series diluted...good” is my standard curve? A poor standard curve leads to over or underestimation of an unknown sample’...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...After uncoating, reverse transcription follows the release of the two strands of viral RNA and of the viral...genome can direct the production, packaging, and release of progeny viral particles. And there you have ...

  11. CRISPR Antimicrobials

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    Blog Post
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  12. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ... viral vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...Resources on Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols...

  13. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...easy to export data from the ELN directly into at least one and preferably more than one of these five general...other side of the pond through interacting with colleagues in Edinburgh. You can follow Rory on Twitter ...

  14. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...base in genomic DNA without double-stranded DNA cleavage." Nature 533.7603 (2016): 420. PubMed PMID: 27096365...editing of A• T to G• C in genomic DNA without DNA cleavage." Nature 551.7681 (2017): 464. PubMed PMID: 29160308...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for. The result is redundancy...organism. So what are the evolutionary constraints that lead to these preferences and what can we do about them...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    ...ticker tape By Brian O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional...Application section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal when tested... gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...understand isoform-specific gene regulation — at least, more straightforward than generating cDNA for each...

  20. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...optimization within your experiment. Read on to learn more about the various controls that can be used...biological controls is independence: at the very least you should repeat your whole experiment from start...
Showing: 801 - 820 of 1017 results