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  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For...PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. ...

  7. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...choosing a MoClo strategy and kit if you want to learn more about this cloning technique! Many thanks to...

  8. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...

  9. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...

  10. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes in live animals...Addgene Blog Tips for using FRET in Your Experiments Learn about Fluorophore Targeting for FRET Studies FRET...

  11. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish ...Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous...

  12. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. What is fascinating... to visualize changes in gene expression and may lead to the development of a whole new range of methods...

  13. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... to admit to inviting interesting friends and colleagues to events for this purpose. If possible, make... more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30 if discussion...

  15. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize axonal projections and putative...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...applications that don’t yet exist, but it wasn’t clear that this was even possible. There is a variety ...that MagLOV’s “astonishing” magnetoresponse was clearly visible whether expressed in E. coli, in mammalian...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...appear as opaque dots (colonies should contain at least 50 cells and be well-separated. If the growth rate...to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with different levels...

  19. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...selectively is especially essential. If you want to learn more about how viruses can be used for chemigenetics...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle...PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation Catch...
Showing: 781 - 800 of 1017 results