...trajectories and identify both up- and down-regulated pathways. Individual cellular fates were then matched with...valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation...

... in organoid cultures are stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This...Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

...be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’s likely someway...networks, and selectively activating signaling pathways. Many scientists came to the Addgene booth to ...

...endogenous RNAi pathways. These mutations however can interfere with other genetic pathways making it difficult...

...general repair pathways: the efficient but error-prone non-homologous end joining (NHEJ) pathway and the less...occurring repair pathway and is the primary repair mechanism for DSBs. While this pathway is efficient in...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template...involved in NHEJ or upregulating those in the HDR pathway can also help. Additionally, constructs with Cas9...fused components that promote the intended repair pathway or inhibit unwanted ones and improve editing efficiency...However, they promote the base excision repair pathway, which fully removes the uridine from the DNA and...evolved to excise inosine to access the same repair pathway and convert A to any other base. In principle, ...

...To control for protein degradation due to other pathways, it is standard practice to compare degradation...ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active form of...

...earning their PhDs (3), showing that the ‘standard’ pathway into academia may in fact be just another ‘alternative...recently launched a survey “Identifying Career Pathways for PhDs in Science”, through which she collects...

... in different steps in the glycan biosynthetic pathway and have a wide range of clinical manifestations...lines to study how immune processes and biological pathways are altered in PMM2-CDG. These studies are expected...

...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

...further, his lab is now studying how Argonaute pathways may be involved in preventing silencing of self-genes...self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive...

... using the endogenous homology directed repair pathway, a new user-defined DNA sequence (like GFP) can...previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a...

...associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein might...undergraduate research experiences (CUREs) as a pathway to diversify science. Evolution and Development...

... exploiting the homology-directed repair (HDR) pathway which occurs in cells at low frequencies and relies...A, A>G, and T>C) installation by base editors. Pathways that compete with the desired outcome are not ...

...since essential and non-essential genes for both pathways had previously been identified (Hart et al, Bassik...systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility." Cell 152.4 (...

...repaired by the nonhomologous end-joining (NHEJ) pathway, leading to nonspecific nucleotide insertions, ...homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously...

...and more, often driven by Rho family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs...phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural head groups for applications...

...genome editing. The homology-directed repair (HDR) pathway is downregulated in mature tissues - no problem... through the non-homologous end joining (NHEJ) pathway. It’s estimated that very little dystrophin correction...

...silencing pathways: transcriptional and post-transcriptional gene silencing (PTGS). In either pathway, short...

...approved monoclonal antibodies targeting the PD-L1 pathway, VEGF production, and CTLA4 and CD protein blocking...Research Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ...

...body, or they can be used to activate cellular pathways by binding to cell surface receptors. These applications...are typically used for investigating signaling pathways or developing therapeutics. Experimentally, antibodies...