...Research mTOR Pathway mTOR Pathway You May Also Like: Cancer Pathway ORF Kit Ras Pathway p53 Pathway Tackling...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic...promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids...generated with the help of David Sabatini . mTOR Pathway Color is used for clarity and does not indicate...the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on a name to find available plasmids...regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini & Wood) Tackling Cancers’ Drug...

...Research p53 Pathway p53 Pathway You May Also Like: Cancer Pathway ORF Kit mTOR pathway Ras pathway Background...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription factor and tumor...mutant p53 is known to function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids...does not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate...generated with the help of . Return to top p53 Pathway - Gene List Click on a name to find available plasmids...Recruitment of p300/CBP in p53-dependent signal pathways. Avantaggiati ML, Ogryzko V, Gardner K, Giordano...

...Research Ras Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small GTPase and is ...conditions GTPases play prominent roles in signaling pathways regulating cell migration, survival, adhesion,...and all tissues. Ras can activate a variety of pathways, which ultimately turn on genes involved in cell...constitutively active form, constantly activating pathways for cell growth and potentially leading to cancer...found in around 20-25% of all human cancers. Ras Pathway Plasmids Click on a name to find available plasmids...

...signaling pathway, focusing on the main pathways involved in cancer development. These pathways are important...their pathway dependencies are extremely important for classification and treatment. On each pathway page...signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier Lab RAS Pathway Clone Collection...plasmids for cancer research, organized by signaling pathway. Special...of interest. Browse Cancer Research Plasmids by Pathway mTOR mTOR is a protein kinase that anchors a signaling...types as part of a “pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & ...Life Technologies™) to permit construction of RAS pathway gene expression vectors for in vitro and in vivo...

...yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs) Jonathan Ting Viviana... Cre recombinase expression in striatal direct pathway D1-MSNs Jonathan Ting Viviana Gradinaru 199777-...Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru 37825-CAP-B10...cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan Ting Viviana...CN4033) Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana... Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana...

...biosensors can enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving...calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29. Baljit Khakh...imaging. Front Mol Neurosci. 2013 Sep 24;6:26. John Garthwaite cGMP (cyclic GMP) FRET-based cGMP biosensor SponGee...Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring...pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier...Synaptophysin with tandem repeats of pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle...dynamics. Nature. 2010 Jul 8. 466(7303):263-6. Martin Schwartz Tension Talin tension sensors Talin tension sensor...

...Finger Consortium Pathways & ORFs Plasmids are grouped together as part of a pathway or ORF. MAPK Nuclear...Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries Pooled...Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease...

...general repair pathways: the efficient but error-prone non-homologous end joining (NHEJ) pathway and the less...occurring repair pathway and is the primary repair mechanism for DSBs. While this pathway is efficient in...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template...involved in NHEJ or upregulating those in the HDR pathway can also help. Additionally, constructs with Cas9...fused components that promote the intended repair pathway or inhibit unwanted ones and improve editing efficiency...However, they promote the base excision repair pathway, which fully removes the uridine from the DNA and...evolved to excise inosine to access the same repair pathway and convert A to any other base. In principle, ...

...Parkinson's Jens Schwamborn 85847 pDONOR-SNCAe3-WT SNCA CAG Parkinson's Jens Schwamborn 85848 pDONOR-SNCAe3...Parkinson's Jens Schwamborn 86154 pDONOR-PARK6e5-I368N PINK1 CAG Parkinson's Jens Schwamborn 87085 pCMV4-ApoE2... pRS-45 TBP His, yTAND12 T7 Parkinson's Harald Schwalbe 40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's...CMV-CRY2oligo-mcherry-ANXA11 ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz 164204 CMV-CRY2oligo-mcherry-ANXA11(D40G) ANXA11...ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz 164205 CMV-CRY2oligo-mcherry-ANXA11(R235Q) ANXA11 mCherry...mCherry CMV ALS Jennifer Lippincott-Schwartz 164206 CMV-CRY2oligo-mcherry-ANXA11(R346C) ANXA11 mCherry...mCherry CMV ALS Jennifer Lippincott-Schwartz 164207 CMV-CRY2oligo-mcherry-ANXA11(1-185aa) ANXA11 mCherry CMV...

...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization of a protein... Many of the proteins that enter the secretory pathway require chaperones to help with folding or post-translational...search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene...

...to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux Pumps...Synthetic Biology plasmids for metabolic enzymes and pathways. Plasmid...

...different sites in various endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The expression... pMLM3613 ). Additional details can be found in Hwang & Fu et al., Nat Biotechnol. 2013 and users are ...

...different target sites in endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The Cas9 expression...expression of Cas9 in zebrafish can be found in Hwang & Fu et al., Nat Biotechnol. 2013. The guide RNA...

...egularly I nterspaced S hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform, to repress...mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , Hwang et al. ), is that the nuclease-deficient mutant ...

...fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8):1117-1131. Parmar...modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into...

... a microbial pathogen, they trigger signalling pathways that lead to the activation of an immune response...PIG4, SAA, TP53I4 SAA2 serum amyloid A2 - SBDS Shwachman-Bodian-Diamond syndrome CGI-97, FLJ10917, SDS,...of alpha-syn(A53T). Target Accelerator Genetic Pathway Reference Set : From Jesse Boehm , William Hahn...ORFs that represent over 25 cellular signaling pathways and processes. Addgene Blogs Adeno Associated ...

...University of California, Santa Barbara Martin Schwartz , Yale University Stephen Sligar , University ...

...efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung ...

...engineered cellular receptors that affect signalling pathways within cells. Subcellular Localization Determine...

... Publications Kaufman ML, Goodson NB, Park KU, Schwanke M, Office E, Schneider SR, Abraham J, Hensley ...