...hand, if a repair template with homology arms was supplied, the break could be repaired according to this...

...that I was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science...seems to break everyday. Many researchers and supporters of science marched to advocate for scientist ...things work, test theories, and solve problems is supposed to be impartial. The best science happens when...°W – Vanessa Sung Hundreds of scientists and supporters in Montreal took to the streets on April 22 to...when I saw that there was going to be a march in support of science, I loved the idea and decided to go....Albany, Rep. Paul Tonko also came and spoke in support of science - he's one of the few members of Congress...science showed up to march. We had full support of local law enforcement, and, although there was at least...

...milestone. Viral service supporters We would like to thank the companies who supported this project with funding...seed money was used to build a completely self-supporting service so that Addgene is not dependent on grants... in accelerating the Viral Service. With their support we are able to provide faster access to a larger...number of viral preps. Visit our Sponsors and Supporters Page Thank you to all of our generous, sharing...Addgene repository. Without these scientists and the support of their technology transfer offices, Addgene would...Jonathan Ting’s lab from the Allen Institute have supported the AAV initiative from the beginning and have...Finally, we are blessed to have an incredibly supportive Board of Directors at Addgene. Our Board helps...

... The success of these projects allows us to support more new projects. Diversification is happening... quality and success. Customer and technical support Our customer and technical service brands continue...sharing, but we don’t have to worry about getting support from depositors. We regularly hear how much they...blessed with a large community of scientists who support us by depositing, requesting and spreading the ...Addgene Board of Directors is mature and poised to support our growth. Thanks to Joanna Brownstein (our outgoing...and Dave Root (incoming Chair) and our entire supportive Board, we have a robust committee process in ...thank them wholeheartedly for their service and support. The Addgene management team leads with excellence...

...which we talk about below. Processing The viral supernatant is typically collected over the course of a couple...to 24 hours. Processing the virus-containing supernatant will depend on the virus type. AAV is typically...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any...than a P1 virus. Pro-tip! Centrifuge the viral supernatant in between passages to eliminate dead cells and...day or two, but unlike mammalian systems, the supernatant isn’t collected sequentially. This is because...majority of cells have died to collect your viral supernatant. Baculovirus replicates inside insect cells, ...ends in cell lysis, releasing the virus to the supernatant. The virus will reach peak production when roughly...

...Dermal Fibroblasts Neurons Lentiviral Human REST suppression mediates neural conversion of adult human fibroblasts...

...t always indicate the presence of supportive data. To be supportive, the data must show that the antigen...This is the most usual level of evidence supplied to support the suitability of an antibody for its target... antibodies. It is also important to seek out supportive data on candidate antibodies from the manufacturer...a specific cytokine) known to either induce or suppress the expression of a target of interest, you can...However, the cell treatment will frequently induce/suppress the expression of many related proteins, not just...protein, show the same pattern of labeling, this is supportive of their specificity for the protein. A challenge...open science ecosystem. It has received in-kind support to produce this data from commercial antibody manufacturers...

...hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the data, scientists...trust in scientists if the march highlights data supporting unwanted results (14). Some loss of trust, I ...science) they come from many different places supporting many different positions. It would be detrimental...going change depending on the number of people supporting it.  3) Linking scientists to one particular ...world. There is no requirement that marchers or supporters be scientists – on the contrary, anyone and everyone...satellite marches. Anyone can join the marches or support the cause. For science to be integrated into politics...29). I tend to agree with him. Information on supporting scientists and science in government: 314 Action...

....   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero fan. This year...Cambridge Super Hero 5K on October 23rd. This is a great event at which people dress up like superheroes or ... their power from the emotion HOPE; a fitting superpower for those running on behalf of rare disease sufferers... superhero 5k...

...arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and information across...AAV vectors, as well as developing and sharing supportive educational resources. By 2017, we had distributed... the COVID-19 pandemic, and Addgene pivoted to support researchers combatting the virus. Our COVID-19 ...Addgene began to think about how we could expand our support for the research community once more. Our ready-to-use...another plasmid-based product that, with Addgene’s support, could fill a community need for high-quality service...Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also created a .... And we look forward to twenty more years of supporting you in your work, your research, and your discoveries...

...which the required adenoviral helper genes are supplied on two different packaging plasmids: the pHelper...your cells, wait 2–5 days and then collect the supernatant and/or producer cells for further processing....you can consider using unpurified AAV from the supernatant (Goodwin et al., 2020) or crude cell lysate (...  Purification To purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG)... 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration of 8%). If, however, you...sonication.  The obtained virions from cells and supernatant then need to be purified by density gradient ...particles containing a viral genome. While ddPCR is superior in precision and variability, qPCR is the more...

...benefit from some Addgene support? Please get in touch, as we aim to support a couple of events a year...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes...involved with. The society provides talks and career support for a range of different scientific disciplines...alumni to mentor younger students in society and to support iGEM alumni-industry networking to promote the ... able to help with every request, we do aim to support events and scientific community activities throughout...

...nanometers of the glass/water interface. Super resolution microscopy Super Resolution Microscopy allows for imaging...matter whether you are a sports photographer at the Super Bowl, a medical technologist taking an x-ray, or...spatial contrast (i.e. optical sectioning (2) or super-resolution (3). Fluorescence microscopy techniques...higher signal to noise ratio can be achieved (6). Super-resolution techniques such as Structured Illumination... these types of samples. Prior to the advent of super-resolution imaging, the light microscope was limited... appear as a fuzzy 200-300 nm spot when imaged. Super-resolution microscopy uses a number of optical and...

...10 that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by chemical...immune cells and cells of epithelial origin. Suppressing function, creating tolerance, and evading recognition...by which tumors sidestep immune surveillance: Suppressing Function Antigen presenting cells (APCs) that...motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive immune cells that restrict the production and... change CD4+ Th cells into regulatory cells to suppress function. Creating Tolerance T-regs in the tumor...combination of tolerance marker blockade and suppression of tumor-aiding cytokines, for example, will ...

... people who will support your professional development I had a wonderfully supportive mentor and graduate...they had my back. If your advisors are not as supportive of your career goals, find people who are. I ...mentioned, my graduate office staff was amazingly supportive. I talked to them about my interests and my ideas...professional organizations, and other nonprofits to gain support for CINSO. For instance, I tweeted to the Indiana...ever since, the State Museum has been the best supporter and most productive partnership CINSO has had....

...the scientific support team at Synthego. Her work centers around educating and supporting novice to expert-level...was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step ...this problem. This tool was initially created to support the CRISPR analysis needs of Synthego’s scientists...other features and functionalities, please email support@synthego.com. Overview of ICE editing analysis ...CRISPR Gene Knockout Kit. Please contact us at support@synthego.com if you have any questions, and check...

...Singletons together would provide a structure and support network for people attending large meetings. A ...those who could not participate but provided moral support was invaluable.  Mixing metaphors and scientists...societies and conferences should strive to provide support to early career scientists, students, and those...ALL their members.  Shoutout to our mentors and supporters Thanks to Dr. Amanda L. Gunn  and Dr. Denise ...Singletons is especially grateful to its mentors and supporters during this first year. Mentors Amanda Gunn, ..., and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander,  Jill Bradley, Taylor...

...scientists. Many members of the scientific community support open sharing. But, one critical part was missing...universities are showing an unprecedented amount of support about allowing use of plasmids for research by ...weeks. As of June 2020, we are happy to have the support of the following organizations to share materials... synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire Ginkgo ...and funding initiatives such as Fast Grants that support us in our mission.  While rapidly expanding our...

... world. To support this pace of research, new, centralized databases and centers to support the sharing...like here at Addgene!), and new databases are supporting their access to useful resources during this ...research Universities have also been stepping up to support COVID-19 research. The new Center for COVID-19 ...Preparedness (CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data...

... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...well as developing yeast as source for dietary supplements! Development of a bacterial expression system...plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini Nair, University of Baroda, India...proteins in order to manage insect pests and to support sustainable agriculture. For this project the expression...in Mexico. At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...