Skip to main content
Addgene
Showing: 61 - 80 of 253 results

  1. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

  3. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved...

  4. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  6. Plan Your Experiment

    Type
    Collection
    ...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific ...

  7. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...apart; however, they still remain attached to the ciliate. It’s thought that they function as a kind of armor...Defensive extrusive ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are...

  10. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...develop the web page. The team manager must then facilitate appropriate communication between members of ...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone...

  11. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...antibody-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing Monoclonal...doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing Monoclonal...

  12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ... space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9 multiplexing in systems with limited...overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such as noncoding...

  13. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this...protein PEG10 specifically binds its own mRNA to facilitate its packaging into virus-like particles. Feng...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...detect expression of toxin mRNA from Clostridium difficile. As mRNA expression cannot be identified with ...infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed ...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    ...to start an open science nonprofit that would facilitate the sharing of plasmids between scientists. The... r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process, which...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    ... broaden the conversations about genetics and facilitate dialogue between scientists and communities. ...With our focus on engaging the community and facilitating dialogue, a major part of our everyday work ...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    ... you will be doing your research in a group or facility that is a world leader in your chosen area of ...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

  19. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...There are several facilities nationwide where you can obtain excellent quality, high titer rAAV (including...like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The most common production...
Showing: 61 - 80 of 253 results