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  1. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  3. How Addgene Creates Content

    Type
    Blog Post
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...two Jack Russell Terriers. More SynBio Reading: Learn more about Revolution Bioengineering Follow RevBio...

  7. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...selection markers.  The yeast origin of replication (Please note: This first section primarily pertains to ... used for color and growth selection if divalent lead ions are used in the growth media. ura4+ pyrimidine...

  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect, perhaps...give advice for your interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include...

  10. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org...

  11. To Each HIS Own

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    Blog Post
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in order...

  12. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, ...PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins...

  15. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...cell line. For more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral...

  20. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... engineered mice in your experiment. As you’ve learned in Part 1, there are many types of genetically ...about Cre-lox Read our Genome Engineering Guide Learn more in our CRIPSR guide ...
Showing: 761 - 780 of 1016 results