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Showing: 741 - 760 of 991 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  2. Plasmids 101: Environmental Plasmids

    Type
    Blog Post
    ...from and why do they exist in nature? Read on to learn more about environmental plasmids, and how they’...plasmid tools available in the Addgene repository. To learn more about some other common plasmid features such...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

  4. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...

  5. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city...

  8. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ...

  10. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ...

  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...said. “We also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe...

  14. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert with Fluorecent...Post-translational Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible ...

  15. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

  18. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...

  19. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...
Showing: 741 - 760 of 991 results