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Showing: 741 - 760 of 1017 results

  1. RUBY-Red Siliques

    Type
    Blog Post
    ...segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ...

  4. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...sometimes up to millions of new copies, which are released from the cell like baby spiders from an egg sac...our Viral Vectors 101 eBook, out if you want to learn more. References and resources References Balloux...

  5. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur at...First impressions are everything and you want to leave people remembering your potential as an entrepreneur...

  7. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR...

  8. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...

  14. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ...

  15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...said. “We also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical...

  16. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe...

  18. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  19. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert with Fluorecent...Post-translational Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible ...

  20. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...
Showing: 741 - 760 of 1017 results