...last, homework assignments. The instructors are leaders in the field of microbiology with multiple high-profile...groups, we were required to critique two of our colleagues’ manuscripts and discuss changes to particular...

...for scientists to understand how GPCR activation leads to downstream effects.  Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated...

...our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection...

...manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze....  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker can help you ...

...targeted RNA knockdown, which leads to unique non-specific RNA cleavage in bacteria. In mammalian systems...

...also drawn out as drugs can remain present until cleared by the body. Administering the drug once can result...vectors? Browse our Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources...

...work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission to make ...Other Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive...

...repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released transposon can now...

...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...choosing your perfect plasmid backbone. Or just ask –  leave a message in the comments below and an Addgenie ...

...the Cas9 nickase which will together introduce cleavage of the target site with efficiency similar to ...system, including one recently from the Zhang group. Learn more here. Q2: When designing oligos for cloning...

...cycles will keep most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast...the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your...

...relevant variants. This list is not exhaustive - please browse our curated list of opsins here to find ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...protein in the light ~400 (on)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible...

...against the variants? Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard...Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org...

...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids...

...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR...

...sometimes up to millions of new copies, which are released from the cell like baby spiders from an egg sac...our Viral Vectors 101 eBook, out if you want to learn more. References and resources References Balloux...

...segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ...

...ever like to write about a recent plasmid deposit please sign up here.  Human whole genome sgRNA iBAR ...calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase that...

...Association and it was through this connection that she learned more about Addgene. “The mission to share critical...and Ear and Harvard Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has...

...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur at...First impressions are everything and you want to leave people remembering your potential as an entrepreneur...