...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...path you want to follow, check out the distance learning courses at the National Council for the Training...

...Washington DC; convening meetings of experts and leaders from academia, industry, community, education and...the biological sciences, as well as to acquire leadership and communication experience. These included ... science career path Resources on Addgene.org Learn how Addgene engages with the community on our Addgene...

...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

...one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...these criteria and deemed positive, it will be released through the website for public reference. The ... sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant...

...Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling...ECFP C155 of ECFP pairs with N173 derived from Cerulean or Venus Identification of new fluorescent protein...

...analyzes publications and figures using machine learning to extract important features such as influential...you organize and search for papers later. Pro tip Learn more about authors by exploring author pages (for...literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter Read our Early...

...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...to read. The key to the success of this style is clear borders around your columns, and good spacing between...used for displaying all of one’s figures, or at least the majority of the visual data. This central box...

... “Download Deposit Spreadsheet” (see Figure 1). Please read our “How to Deposit Your Plasmids with Addgene... a collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits...37 °C, or room temperature. Growth Strain Please indicate which growth strain Addgene should propagate...

...Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has...Lineage Tracing Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral...

...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...

...preparation of the fiber optics is probably the least difficult portion of optogenetics (though it does...polishing. Efficiency of the implant should be at least 85% of the initial laser output. The same considerations...University.  His interests include the role of learning and memory in opioid addiction and science communication...

...account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook... into executing a successful business that the learning curve is pretty steep. But we can rely on one ... about this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun...

...pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your... repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid...Barcode Watermelon Library 5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX...

...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting...fruitful. After the conference Bring back what you’ve learned from the poster session to your lab. Perhaps some...scientific communication, and scientific networking Learn how to set up and informational interview and Expand...

... Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check...the addition of post-translational modifications Learn more molecular biology in our Plasmids 101 eBook...

...FPs to highlight various subcellular structures. Please see Figure 1 for commonly used markers for major...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging ...

...continue to to build and develop this new resource! Please leave us your thoughts in the comments section below...Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer...