... Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina Fan, we created...Production webpage). While the new team took the lead on operationalizing viral production and distribution...Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research...

...’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission, briefly summarizing...house style and prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread...that all required information is provided. The clearer you write your paper and the more information you...

...within the resistant populations were shared by at least one other replicate; prior to treatment, there are...particularly interested in applying what we have learned about tumor heterogeneity to drug discovery and...PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find...

... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...the reader to interpret the results to see if it leads you to the same conclusion. Personally, I like papers...interest. Set aside some time each day or week to at least scan the titles of what’s going on in your research...

...ever like to write about a recent plasmid deposit please sign up here.          Listen to this episode ...Acr activity as a biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive...usually expressed from a different plasmid, which may lead to unequal gene dosage and high variability of protein...

...targets. Check out our post on Biotinylation to learn more about biotin and its use in molecular biology...other classes that we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection... with BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking...

...proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...Fluorescent microscopy techniques - Which is best for me? Learn how small molecule fluorophores can be used for ...

...estradiol for hER or progesterone for hPR - leads to nuclear localization, binding to the appropriate promoter...ever like to write about a recent plasmid deposit please sign up here. No time to read? Listen to our Hot...

...people said it took them more than 22 hours, so at least the better part of four days. How much does it cost... a number of factors, but we can assume it’s at least equal to the $155 spent on reagents. Five ways to...tell. But even from these rough estimates, it’s clear that community engagement with and use of repositories...

... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

...Submission” and “Spreadsheet Submission” (see Figure 1). Please read our “How to Deposit Your Plasmids with Addgene... a collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits...37 °C, or room temperature. Growth Strain Please indicate which growth strain Addgene should propagate...

...Having never been to China myself, I was pleased to be able to participate when the organizers of the...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...Medicine Initiative and how this initiative will lead to advances in human health. It was inspiring to...

...preparation of the fiber optics is probably the least difficult portion of optogenetics (though it does...polishing. Efficiency of the implant should be at least 85% of the initial laser output. The same considerations...University.  His interests include the role of learning and memory in opioid addiction and science communication...

...analyzes publications and figures using machine learning to extract important features such as influential...you organize and search for papers later. Pro tip Learn more about authors by exploring author pages (for...literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter Read our Early...

...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...to read. The key to the success of this style is clear borders around your columns, and good spacing between...used for displaying all of one’s figures, or at least the majority of the visual data. This central box...

...the different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see...that are codon optimized. Browse the collection or learn more about it on the blog. COVID-19 research is ....0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein...

...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...path you want to follow, check out the distance learning courses at the National Council for the Training...