...’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission, briefly summarizing...house style and prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread...that all required information is provided. The clearer you write your paper and the more information you...

...ever like to write about a recent plasmid deposit please sign up here.          Listen to this episode ...Acr activity as a biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive...usually expressed from a different plasmid, which may lead to unequal gene dosage and high variability of protein...

...targets. Check out our post on Biotinylation to learn more about biotin and its use in molecular biology...other classes that we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection... with BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking...

...people said it took them more than 22 hours, so at least the better part of four days. How much does it cost... a number of factors, but we can assume it’s at least equal to the $155 spent on reagents. Five ways to...tell. But even from these rough estimates, it’s clear that community engagement with and use of repositories...

...estradiol for hER or progesterone for hPR - leads to nuclear localization, binding to the appropriate promoter...ever like to write about a recent plasmid deposit please sign up here. No time to read? Listen to our Hot...

... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

...proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...Fluorescent microscopy techniques - Which is best for me? Learn how small molecule fluorophores can be used for ...

...within the resistant populations were shared by at least one other replicate; prior to treatment, there are...particularly interested in applying what we have learned about tumor heterogeneity to drug discovery and...PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find...

... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...the reader to interpret the results to see if it leads you to the same conclusion. Personally, I like papers...interest. Set aside some time each day or week to at least scan the titles of what’s going on in your research...

...Washington DC; convening meetings of experts and leaders from academia, industry, community, education and...the biological sciences, as well as to acquire leadership and communication experience. These included ... science career path Resources on Addgene.org Learn how Addgene engages with the community on our Addgene...

...reprobing, maximize your signal by starting with the least-abundant protein and working toward the most abundant...primary and secondary antibodies from your membrane, clearing the way for the second blot. There are multiple...with the mild one. Reprobe Once your membrane is clean of antibody, you’re ready to repeat your western...

...cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic potential...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...Early-stage trials showed a high success rate in clearing misfolded protein levels as measured in the blood...

...receptors hijack cell signaling pathways and can lead to such responses as decreased heart rate (Redfern...properties such as endogenous gene expression, which can lead to changes in normal cell physiology and function...posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide...

...one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...these criteria and deemed positive, it will be released through the website for public reference. The ... sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman is a research assistant...

...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...engineered entirely new types of plasmids without clear analogues in nature. For example, helper plasmids...plasmid tools available in the Addgene repository. To learn more about some other common plasmid features, such...

...analyzes publications and figures using machine learning to extract important features such as influential...you organize and search for papers later. Pro tip Learn more about authors by exploring author pages (for...literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter Read our Early...

...your questions, data, and conclusions should be clearly identified and interpreted in less than a couple...to read. The key to the success of this style is clear borders around your columns, and good spacing between...used for displaying all of one’s figures, or at least the majority of the visual data. This central box...

...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

...Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling...ECFP C155 of ECFP pairs with N173 derived from Cerulean or Venus Identification of new fluorescent protein...

...the different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see...that are codon optimized. Browse the collection or learn more about it on the blog. COVID-19 research is ....0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein...