...such as fluorescent microscopy must be used. To learn more about the most suitable fluorescent microscopy...structure of the protein. In some cases this can lead to changes or a complete loss of protein function...more autofluorescence and nonspecific staining leading to false positives. Numerous dyes can be used for...Check out other methods using fluorescent proteins Learn more about antibodies Resources on Addgene.org ...

...epifluorescence microscopy wasn’t able to give a clear view of cellular movement in real time. Two-photon...microscopy has uncovered host-pathogen interactions leading to the understanding of effector function in infected...Weninger. "Dendritic cell behaviour in vivo: lessons learned from intravital two-photon microscopy." Immunology... Get Tips on Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent...

... the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References ...Baby Gel Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources...Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests Make Your...

... company, Science with Tom. In this podcast, we learn more about Tom and pick his brain for advice on ...importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed video about...generation science standards and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com...

...journey is at an end! Their name is...(drum roll, please!)... Abi! Thank you so much to everyone who submitted...Which name best fits our new immunoglobulin friend? Please help us decide by voting for your favorite on this...Original Blugene and Aavery would like your attention, please, as they introduce their new friend…  Fig...

...forward with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course...read through each one, plug in some headphones to learn more about the latest and greatest plasmids through...Plasmid webpage. We hope you enjoy this new way to learn about plasmid technologies. If you have any comments...

...Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ....     Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael...

...Since this is a beta project, it will run for at least 6 months as we assess the needs of the scientific...Browse blog posts from our Plasmids 101 blog series Learn about the different grades of DNA  Read about the...ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our Molecular...

...Cambridge to spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use...Addgene Blog Check Out Posts on Synthetic Biology Learn about the Addgene, Seeding Labs Plasmid Grant Read...

...to manage these changes quickly and harmlessly. Learn much more about the laws and their possible repercussions...School Advice Part 2: Building Community.” This post leads readers to our latest podcast all about graduate...to catch up on ways to use CRISPR in plants, to learn techniques for detecting genome edits, to discover...

... Now, they’ve filled that gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study...tandem. They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully...Currently, the kit only includes two versions of Cas9, nuclease and nickase, but we are now adopting dCas9, dCas9...

...stress-induced biochemical changes that could otherwise lead to depression. And there you have it, mck-PGC-1a1...beneficial effects on the brain,” Ruas said in a press release. “We actually found the opposite: well-trained ... demands,” they write. “In the future, these may lead to treatments for a variety of brain disorders, ...

...corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight). If you never looked...like these…. 3. Expect to get 500-700 bases of clean reliable DNA sequence Anything less and you might...and small, so I suggest designing your primer at least 50bp upstream of the sequence of interest. 5. Use...

...Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 ...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

...Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the Kotton Lab Learn more ...mission to promote scientific sharing. Listen to learn more about Darrell, his research, and his focus ...

...Attendees consistently mentioned how pleased they were to learn about these exciting scientific topics ... members gave up a beautiful summer Saturday to learn about science, but they didn’t seem to mind! Attendees...

...scared of taking risks, afraid of leaping forward but I have learned to push myself to the forefront, ...internship at Addgene. Although my interests are very clear, I had no idea how to translate them into real world...

...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. ...something you’d like to see more of? Let us know by leaving a comment or emailing us at [email protected].  ...

...spreadsheet of random numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library Screening...PMCID: PMC4744125. Resources on the Addgene Blog Learn to Conduct Genome-wide Screens with CRISPR/Cas9 ...

...Science Signaling in December 2014, he and his colleagues at Duke University, in collaboration with “cloning... of the cancer pathways ORFs kit Wood and his colleagues developed the kit for use in screening for pathways...PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been Used in Cancer...