...need to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage...finally grasped just how complicated DNA repair pathways are. I fully admit to being a DNA repair newbie...template strategies activate different HDR sub-pathways like synthesis-dependent strand annealing (SDSA...

...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing... counterpart, enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance, yeast...

...repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences (thus inactivating...breaks repaired by homology directed repair (HDR) pathways can modify the sequence of a gene (thus altering...Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab Members who worked on ...

...potential toxicity and cross-reactivity with other pathways. Since these effectors are diffusible, they’re...input patterns may also be used to engineer new pathways with sophisticated gene expression controls. Most...inducible promoters that could crosstalk with other pathways. They also reduced system leakiness and increased...

... process. The process can range from a defined pathway or reaction to a complex event such as cancer or...acquired a favorable mutation. But if the cellular pathway of interest conveys no significant survival advantage...advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen would be required...

...metabolic engineering, the expression of enzymes in a pathway will ideally be optimized to promote generation... the shuttling of the intermediates into other pathways. In cases where a specific ratio of products is...variability between the different components. When your pathway needs precise fine-tuning of gene expression levels...

...etc., microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor...isolated afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs...testing any number in the Level 2 assembly. These pathways can require a lot of optimization - which is why...

...cell viability and increase protein production (Schwarz et. al., 2020). Harvest and purification At its....2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, ...Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau ...

...several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to restore the DNA after... S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing...coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:. https://doi.org/10.1038...

...vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023) for a review of other targeted...to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually...

...breaks into the homologous recombination repair pathway. Reagents required to perform pha-1 co-conversion...researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more CRISPR tools for C.... . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...

...biosensors can enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving...calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29. Baljit Khakh...imaging. Front Mol Neurosci. 2013 Sep 24;6:26. John Garthwaite cGMP (cyclic GMP) FRET-based cGMP biosensor SponGee...Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring...pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier...Synaptophysin with tandem repeats of pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle...dynamics. Nature. 2010 Jul 8. 466(7303):263-6. Martin Schwartz Tension Talin tension sensors Talin tension sensor...

...Finger Consortium Pathways & ORFs Plasmids are grouped together as part of a pathway or ORF. MAPK Nuclear...Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries Pooled...Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease...

...general repair pathways: the efficient but error-prone non-homologous end joining (NHEJ) pathway and the less...occurring repair pathway and is the primary repair mechanism for DSBs. While this pathway is efficient in...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template...involved in NHEJ or upregulating those in the HDR pathway can also help. Additionally, constructs with Cas9...fused components that promote the intended repair pathway or inhibit unwanted ones and improve editing efficiency...However, they promote the base excision repair pathway, which fully removes the uridine from the DNA and...evolved to excise inosine to access the same repair pathway and convert A to any other base. In principle, ...

...Parkinson's Jens Schwamborn 85847 pDONOR-SNCAe3-WT SNCA CAG Parkinson's Jens Schwamborn 85848 pDONOR-SNCAe3...Parkinson's Jens Schwamborn 86154 pDONOR-PARK6e5-I368N PINK1 CAG Parkinson's Jens Schwamborn 87085 pCMV4-ApoE2... pRS-45 TBP His, yTAND12 T7 Parkinson's Harald Schwalbe 40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's...CMV-CRY2oligo-mcherry-ANXA11 ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz 164204 CMV-CRY2oligo-mcherry-ANXA11(D40G) ANXA11...ANXA11 mCherry CMV ALS Jennifer Lippincott-Schwartz 164205 CMV-CRY2oligo-mcherry-ANXA11(R235Q) ANXA11 mCherry...mCherry CMV ALS Jennifer Lippincott-Schwartz 164206 CMV-CRY2oligo-mcherry-ANXA11(R346C) ANXA11 mCherry...mCherry CMV ALS Jennifer Lippincott-Schwartz 164207 CMV-CRY2oligo-mcherry-ANXA11(1-185aa) ANXA11 mCherry CMV...

...Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins...Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization of a protein... Many of the proteins that enter the secretory pathway require chaperones to help with folding or post-translational...search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene...

...demonstrating that this system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin... M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench, J. G., LaFleur, M...

...to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux Pumps...Synthetic Biology plasmids for metabolic enzymes and pathways. Plasmid...

... will be able to better choose which genes and pathways to target in future small molecule screens. He...coworkers to test inhibitors of a variety of signaling pathways for their effects on the disease. This work will...

...trajectories and identify both up- and down-regulated pathways. Individual cellular fates were then matched with...valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation...