... passes through the machine’s lasers and gives off a signal read by the machine’s sensors. (Usually this...or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate ...variations in fluorescent signals, and how the machine reads/interprets the signals, the axis is instead...populations. This shape is called a ‘gate’; it tells the machine that everything inside the gate is one population...

...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...

...reagents may be sufficient to express the CRISPR machinery. For more difficult cells (e.g. primary cells)...

...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...further differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical...

...Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close proximity with the target protein, ...degraders provide distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest...to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly excited...FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading to rapid...

...Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. 2015. CETCh-seq: ...

...clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter. Primer Validation...

...repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids...

...Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine...et al., 2019). Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed...be specifically and potently activated by pharmacologically selective effector molecules (PSEMs). Higher...

... efficiency and building prediction tools with machine learning (PRIDICT, PRIDICT2.0).   How to apply...chromatin-based prediction tool (ePRIDICT). In summary, machine learning-based prime editing prediction models ...computational biology. His expertise includes applying machine learning techniques to make sense of biology, with...B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime editing efficiency...

...oncology, virology, immunology, microbiology, and pharmacology (many of which are represented in Addgene's ...cell culture over the last few decades some pharmacologists have tended to avoid cell culture in favour...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel ...

...greater than the one that can be achieved using pharmacological or electrophysiological methods (Deisseroth...depression-like behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157...circuits in response to drug exposure." Neuropsychopharmacology 41.10 (2016): 2424. PubMed PMID: 26934955...applications of optogenetics." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van...

...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...

...organelles, such as mitochondria, as well as macromolecules like actin and myosin, and enzymes for transmitter...two pathways also use different cytoskeletal machineries to facilitate transport: retrograde transport...interaction with uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res 93, 144-157. PubMed...

...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

...Bhang, a research investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new...her new role as an investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working... Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology and is particularly interested...

... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...