...identification of potential RGN target sites and to facilitate design of short oligonucleotides needed to construct...

...devised means of co-opting phage genetic systems to facilitate homologous recombination in bacteria. These quirks...of just a single organism, such as Clostridium difficile, could be targeted and preferentially depleted...system could be further refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on...DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials...

...BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study of the live virus. Alternatively...

...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

...efficient mechanism of homologous recombination to facilitate simple gene replacement/mutation. Yeast expression...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...are used in the growth media. ura4+ pyrimidine (uracil) yes - Grow with 5-FOA*. S. pombe  no no *FOA in...

...shared experience for the research community and facilitates scientific collaborations between institutions...

...these approaches have created this resource to facilitate open and free exchange of these expression plasmids...

...strains are ∆ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes... isolate transformed strains on media that lack uracil, but contain methionine. (This allows cells carrying...Call to Action, Washington, DC. 2. President's Council of Advisors on Science and Technology [PCAST] (...

...Addgene’s repository. For long-term storage in our facilities, every sample is transferred to a 2D barcoded...Pir1. The majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along...kept at Addgene and another is sent to a storage facility). The third tube is used to fulfill orders. Breaking...

...with the Cas13a molecule through a uracil-rich stem loop and facilitates target binding and cleavage through...system, Cas9 uses both a tracrRNA and crRNA to facilitate binding and cleavage of target DNA respectively...

...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

... lab harnessed a group of bacterial toxins to facilitate efficient recombineering (Khetrapal et al., 2015...reduce transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation...system (Lazarus et al., 2019). Additionally, to facilitate modification of strains where conjugation may...

...membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid droplets...

...Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed...

...sgRNA expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can be custom designed...

...and a transposase. The piggyBac™ transposase facilitates the integration of the transposon specifically...achieved using the piggyBac™ transposase, which facilitates direct integration of the donor-transposon (carrying...standard homology directed repair template to facilitate the selection of cells containing your desired...

...44251 pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Do you have suggestions...

...delivery has many advantages for research. Viruses facilitate the delivery of genetic information to hard-to-transfect...

...develop better strategies for combating cancers. To facilitate innovative cancer research, the Reference Reagents...express the integrated gene. These vectors contain uracil-excision based (USER) sites, flanked by ADH1 and...by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target genes (20-40x greater...compensating for histidine (HIS3), leucine (LEU2), uracil (URA3), methionine (MET17), and lysine (LYS2) deficiencies...