...applications was still very new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the...influences in our environments. We are able to react and learn. All of this requires genes to be regulated dynamically...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...processes and disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience...

...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights...section to make this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding...(check out our interview with Michael Koeris to learn how his company uses phage to test for Listeria ...posts in chronological order here: Four Things I Learned at the 10 Years of iPSCs Symposium Tips for Writing...

... well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability...Who even regulates them? You may be surprised to learn that it hasn’t even been 50 years since the creation...fluidic machines handle biological samples and are cleaned between each use. You wouldn’t throw away your ...series!   Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe...

... passion, and patience are required to build and lead a productive and successful research lab. Once goals...champagne, that's a given.” Furthermore, anyone leaving the lab receives send-off via a gift, card, and... the afternoon during the child-rearing years, learning “lots of interesting things from children, their...their classmates, and parents”. He also cooks and cleans with his family, “sometimes listening to audiobooks...

...functionality can be found in the following video: Please remember that the accuracy and reliability of our... plasmid is missing from our collections pages, please shoot us an e-mail at [email protected]. We’d love...visibility in a few other ways. Every quarter we release a Newsletter where we spotlight some of the plasmids... in the repository. And last, but certainly not least, we often feature new plasmid technologies on our...

... also have the option to “Leave us a message.” When you click on the "Leave us a message" button shown...lab’s materials and requesting them from your colleagues, to intellectual property rights and handling...the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources...

... you want. But how do you know if a masters will lead to a career you will like? Here’s two things to ...setting up an informational interview with them to learn how their master's degree prepared them for their... help with manuscript and grant preparation, and lead your own research project.  3. Do you prefer being...eBook.   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now...

...hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR ...

...insights discoverable. Watch this quick video to learn how ReFigure works and continue reading for more... and again as they don’t save the insights they glean from a reading session. To remember what happened...reach the ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering...video to share your science at We Share Science Learn more about Data Sharing Resources at Addgene.org...

... year. The society facilitates opportunities to learn about Synthetic Biology and exciting new technologies...network of fellow scientists that you can grow with, learn from, and mentor while pursuing your research interests... which would benefit from some Addgene support? Please get in touch, as we aim to support a couple of ...Networking Get Career Advice from Addgene Bloggers Learn How to Get Involved in a Profession Organization...

...more years with a small number of formal advisors learning how to be a scientist. It is shocking how little...important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be the kind...science career. Will you be given opportunities to learn and practice successful grant writing, lab budgeting...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if...

...genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted to elucidate...CRISPR loci to generate RNA, which guide a Cas nuclease to the specified exogenous genetic material next...within a host cell. A few virus particles have learned to circumvent the CRISPR/Cas defense by generating...mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3). Surprisingly, few ...

...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes....2019.276 Additional resources on the Addgene blog Learn more about basic viral vector elements Read about...about four considerations for the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression...

...indicated that a reasonable rule is to require at least 96% sequence identity when detecting a reference...commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we dug into... harness common feature detection in a new way, please contact us at snapgene.com. Many thanks to our...Blog Read about our SnapGene powered plasmid maps Learn how we use SnapGene during the plasmid quality control...

...few quirks specific to the PCR cloning process - please check out that post if you need a more detailed...PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the... existing software (a quick internet search will lead you to here and many others). If we put the sequence...product. Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things...

...transferred between cells. At the time, these extranuclear agents of heredity were thought of as everything...first plasmid “cloning” experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101...topology, and cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook And now...and more pictures and pro tips.  Happy reading! Learn more about the history of plasmids and cloning: ...

...transduction.) Compared to CCP transduction, iTOP is at least four times more efficient in primary fibroblasts... gRNA, which subsequently enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo...expression is by nature transient, which may lower nuclease toxicity or the potential for off-target effects... Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat...

...it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent problem... perform an optimization-scale infection and at least one screening-scale experiment achieving the gRNA...the publications associated with these libraries. Please note, the pooled libraries we distribute in lentivirus...or are thinking about using our viral services. Please send any questions or comments to help@addgene....

...night of the iPSC symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George... of ethics can be daunting because they have no clear cut answers, I applaud the stem cell community for...Disease; and Kevin Eggan presented work that is leading to promising clinical trials for ALS.   I have ...that!  Additional Resources on the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read...

...interesting at an event. Or, perhaps you have a colleague who seems fun and helpful who you’d like to get...connections. I mention this because I want it to be clear that it is completely impossible for me or anyone...contacts over the years in many different ways. This leads me to my last tip: It’s usually not worth connecting...networking Attending a conference soon? Get tips here Learn about informational interviews ...