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We narrowed to 1,026 results for: LEA

Showing: 461 - 480 of 1026 results

  1. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... from Robert Hurt and Mohamed Soufi. So you’ve learned about the Modular Cloning (MoClo) system and what...time to sit down, plan your cloning, and have a clear vision of the final vector structure you want to...), which recognize asymmetric DNA sequences and cleave at a short distance from their recognition sequence...the MoClo system, are an invaluable resource for learning about MoClo in an in-depth and easy-to-understand...Preparing reagents Keep it cool You’ve done all the learning and planning, and your MoClo kit has just arrived...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    ...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...older than those available from biorepositories leading to genotypic and phenotypic artefacts. The life...commercial repositories and the same cell line from a colleague?”. The main difference is the QC and validation...usually the case if you obtain a cell line from a colleague. Time and money are wasted, papers are retracted...

  3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...remains unclear whether the FP misfolding impacts fusion protein function and this could lead to under...localize [4]. Most investigators are surprised to learn that FPs are often poorly suited for cellular compartments...glycosylation, disulfide bond formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible... dark fluorescent proteins in your experiments, please consider the following: How can one ensure that...

  4. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...bit of a steep learning curve and many opportunities for mistakes. The easiest way to learn optogenetics... is an approximation of reward or the sense of pleasure that the animal perceives through taking a drug...University.  His interests include the role of learning and memory in opioid addiction and science communication...Kheirbek, Mazen A., et al. "Differential control of learning and anxiety along the dorsoventral axis of the...

  5. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    ...Bench series was born from the shift to remote learning due to COVID-19. We had many protocols for plasmid...details about personal protective equipment to learning about how to use a tissue culture hood and other...other lab equipment, we’re here for you as you learn the ropes! All material can be found on our Protocols...Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists!   ...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...warm meal on a winter day, you should be happy to learn that many DNA-based reagents are named for soup ... and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative, but...regions in the 5’ UTR of mRNA that bind metabolites, leading to conformational changes that control the expression...the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango, binds to ...PMC4143336. Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate other...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...the uses of biotinylation in molecular biology! Learn about in vivo biotinylation of bacterial fusion ...biotin-avidin complexes bound to your protein of interest leading to amplification of the reporter signal and easier...microenvironments. There are also reversible and cleavable biotinylation reagents to help with the specific...vivo biotinylation of bacterial fusion proteins Learn how biotin is used in Split-BioID: An improved method...method for studying protein-protein interactions Learn how biotin can be used with CRISPR Additional resources...

  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...investigating HIV integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous variants...However, IDLV genomes are transcriptionally active, leading to transient expression of gene products (Wu et... insertional mutagenesis. This integration could lead to an undesirable interruption of a critical gene... off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822...PMID: 18688286 PubMed Central PMCID: PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H....

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...which is then cleaved by RISC and degraded. In the miRNA pathway, RISC doesn’t always cleave the mRNA — ...hairpin shRNA structures that are processed by a ribonuclease called Dicer. In a great simplification of the...protein is what binds the small RNA and either cleaves the target mRNA or recruits other silencing proteins...no tissue specificity, so in some cases they can lead to toxicity and off target effects. More recently...

  10. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation Improves plasmid...cell mcrA, mcrBC Inactivation of pathway that cleaves methylated cytosine DNA Allows for uptake of foreign... mrr, Δ(mcrC-mrr) Inactivation of pathway that cleaves methylated adenine or cytosine DNA Allows for uptake...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...

  11. We’re Going Viral This Week!

    Type
    Blog Post
    ...blog posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about... serotype, injection site, and incubation time. Learn more about the Data Hub in this blog post. Alongside...attending SfN2019, stop by booth #2163 to say hi and learn more about Addgene’s viral service. Additional ...

  12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... the Addgene Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris...needs. For more context, exposition, and detail, please refer to the paper. Why might one be interested...uploaded to Addgene. We also recommend inserting self-cleaving hammerhead ribozymes directly upstream of the ... transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described in Nielsen...

  13. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ... I had found my dream job, because one thing I learned in grad school was that scientists have amazing...at their lab benches. It’s these exchanges with leading scientists (and our growing collection of 25,000...sciences research for the past decade. We’ve also learned along the way that a connected and collaborative...scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical tips...

  14. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...Health and Allied Sciences in Ghana. Read on to learn more about doctors Bengyella and Duedu and how they...tumors is “a major public health problem, and a leading cause of hospitalization for gynaecologic disorders...Program Additional Resources on the Addgene Blog Learn about Plasmid Awards to Researchers Working on Rare... in China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International...

  15. Antibodies 101: Validation

    Type
    Blog Post
    ...even to not bind to their target molecule at all, leading to inconclusive results, erroneous conclusions,... that your antibody has been validated using at least one of these strategies. Choosing which strategy...) the protein of interest in your sample, should lead to reduced or eliminated antibody signal. If you...translation start sites or alternative splicing that could lead to some form of the target protein to be present... Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    ...alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces. Speed...Manitoba due to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are...vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune response. Any investigator who ...antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The most attractive potential...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...LTRs) has been removed, Rev (which facilitates nuclear export) is expressed from a separate plasmid, and...mutation or recombination with human retroviruses could lead to RCL. Regarding transduction efficacy, no differences...Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant and...retrovirus packaging cell lines to avoid recombination leading to helper virus production." Molecular and Cellular...5 Tips for Trouble Shooting Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral...

  18. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ... change? Addgene has you covered. Today we’re releasing 11 bold science-themed backgrounds! Show off your...out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! ...Addgene are shipped as bacterial stabs? You can learn about what format your material will come as (and...molecular biology tools in your research or not! And learn more about the variety of careers available to scientists...

  19. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...countries. We, like everyone around the world, are learning to navigate the changes brought about by the pandemic...opportunities. I have spent the last 9 months quietly learning about Addgene’s past and present, but the real...successes to reflect upon, a wealth of history to learn from, and a compelling future to plan in the changing...people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger scientific...

  20. Science Communication for Everyone

    Type
    Blog Post
    ... guide Learn about different careers in science communication Resources on Addgene.org Learn how Addgene...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com.... Sadangi is an eLife ambassador, SfN community leader, and an early research career panelist at BioTechniques...
Showing: 461 - 480 of 1026 results