Simplify Cloning with in vivo Assembly
Type
Blog Post
...achieved in vivo using a bacterial recombination pathway that is present even in common lab cloning strains...strains.
This intrinsic bacterial recombination pathway, referred to as recA-independent recombination, ...functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination reported...E. coli strains tested so far.
The use of this pathway as a cloning tool was first reported over 25 years...et al., 2016) uses the bacterial recombination pathway to allow any cloning procedure to be performed ...