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We narrowed to 261 results for: Mac

Showing: 21 - 40 of 261 results

  1. Viral Vector Guides and Plasmids

    Type
    Collection
    ...cells in an organism and use the host cellular machinery to produce more virions. B) Viral plasmids have...to packaging cell lines and use their cellular machinery to generate viral vectors for research and gene...genome into the cytoplasm and hijacks the host’s machinery to produce more viruses. In this way, viruses ...

  2. Antibody Guide

    Type
    Collection
    ...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...direction based on the emission(s) read by the machine. Controls for cell sorting methods Flow cytometry...overlap to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674(20...Superecliptic pHluorin pH sensor Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing...cells. Circ Res. 2010 Feb 19;106(3):526-35. Paul Schumacker Redox/ROS Glutathione redox sensors for cytosol...Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based...

  4. AAV Viral Preps

    Type
    Collection
    ...Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida Serotype...

  5. Chemogenetics Plasmids

    Type
    Collection
    ...Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs...respond specifically to small molecules termed Pharmacologically Selective Effector Molecules (PSEMs). PSAMs...

  6. E11 Bio PRISM Collection

    Type
    Collection
    ...tissue, these protein barcodes allow for efficient machine-learning segmentation of neurons and proofreading... microscopy and reconstruction of neurons with machine learning. Adapted from Park & Sheridan et al. (...

  7. mTOR Pathway

    Type
    Collection
    ...proliferation, including an abundant supply of macromolecules and energy, as well as the presence of both...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...

  9. Brain Armamentarium

    Type
    Collection
    ...promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG...promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ... using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal ... using Gateway cloning. Use with 174050 Alberto Macho 136010 psi-CHECK3 Firefly, Renilla Insertion of ...

  11. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Pubmed PMID: 3325793 Schumacher MA (2012) Bacterial plasmid partition machinery: a minimalist approach...origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...they compete for the same replication control machinery within the cell. Most plasmids encode a negative...through inhibiting translation of replication machinery proteins, or directly inhibit replication by binding...origin of replication and blocking replication machinery (del Solar, 1998). As the copy number of the plasmid... structure that physically blocks replication machinery when plasmid copy number is high (del Solar et...replication of each plasmid using unique replication machinery. However, when plasmids have the same origin of...

  12. Deep Dive: qPCR

    Type
    Blog Post
    ...and most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler,...previously-generated validation data, be aware that different machines, samples, and treatments may affect the assay....running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr chemistry is ... by bubbles in wells, variations in volume, or machine surge errors and allow for well-to-well normalization... likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information...information on optimizing for LightCycler machines.) Multiplexing It is possible to multiplex qPCR when using...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ...that you come into the lab wishing there was a machine that could make your life a hundred times easier...off our hands. Good news! There is probably a machine or robot out there that does exactly what you want...lot of time. Enter the Biomek FXP! We wanted a machine that would improve our transformation protocol ...played a factor in our decision to go with this machine, but we also really liked that Beckman was flexible...apart before plating. With this fine piece of machinery we have been able to transform 96 samples in about...lab team. Enter the Liquid Handler!     This machine fits on a table top and can be easily programmed...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

  14. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions... can also download the software for use on any machine with OpenCL enabled hardware. This OpenCL dependency...genomes), then it is worthwhile buying a dedicated machine. On the offline front, there is a Python script...Pasteur, Paris. You can follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al....

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...code of life. In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty’s experiment debunked the myth that...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ... technologies rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation... the use of molecular dynamics simulations and machine learning to develop a predictive understanding ...://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE...

  16. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...

  17. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... passes through the machine’s lasers and gives off a signal read by the machine’s sensors. (Usually this...or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate ...variations in fluorescent signals, and how the machine reads/interprets the signals, the axis is instead...populations. This shape is called a ‘gate’; it tells the machine that everything inside the gate is one population...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...
Showing: 21 - 40 of 261 results