...TALEN technology and using TALEN plasmids... TALENs TALEN Guide Addgene's Guide to TALEN Technologies You ...'s December 2011 Newsletter Overview of TALEN Technology Imagine being able to manipulate a specific region..., zinc finger (ZF) arrays have been the go-to technology for targeting enzymes and other useful protein.... With the template for studying this type of technology already laid by the zinc finger community ( see...DNA regions into a single construct would be technically challenging. Thanks to efforts by the Bogdanove...Addgene. This kit, first described in Nature Biotechnology in August, uses a serial ligation protocol to...

...this technology is used for targeted neuronal manipulation. Practical tips for using the technology in ...Education and Development (AAVED) Series AAV Technologies and Methods for Targeted Neuronal Manipulation...the guide. We hope that this guide will improve technology adoption and scientific outcomes. Event Details...Cepko 2:45 PM Break 3:00 PM (Group Discussion) Technologies and Resources Missing in the Field Kenton Woodard...presentation is to: Give a general overview of the technology and how it can be used for targeted neuronal ...focused on: Potential combination with other technologies to achieve different experimental goals. How... manipulation? Best practices for using this techonology in the lab (main issues/factors that researchers...

...sensors Single-color, ratiometric biosensors for detecting signaling activities in live cells. eLife. 2018...or Cre-dependent) A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo. Neuron...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell Biol. 2018 ...sensor Single-color, ratiometric biosensors for detecting signaling activities in live cells. eLife. 2018...pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose...intracellular tryptophan levels and flux Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine...indicator for in vitro and in vivo studies. Nat Biotechnol. 2018 Sep;36(8):726-737. Yulong Li Acetylcholine...

... genome-wide screening. CRISPR Technological Advances CRISPR technology development has occurred at a ...various biological and translational technologies. Biotechnology companies are exploring therapeutic applications...applications, eclipsing past genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs...Scientists have also developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors... it’s clear that CRISPR is a truly disruptive technology for basic research. The beauty of CRISPR is that...nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574 Fujita T, Fujii.... High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature . 529(...

...Zebrafish ( Sander et al., Nat Biotechnol. 2011 ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells...cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon and Tsai et al., Nat Biotechnol 2012 ) Human pluripotent...Ligation) assembly method (Sander et al., Nat Biotechnol. 2011), the REAL-Fast assembly method (Reyon ...) assembly method (Reyon and Tsai et al., Nat Biotechnol 2012). All three of these platforms assemble ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three methods...pluripotent stem cells ( Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells...

...of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP minus plasmid...by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite library was generated ...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide interactions...interactions (“mode #2” expression, Barber et al, Nat. Biotech. 2018). The phosphosite library is fused to the...kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library ...Gerstein M, Sampson JR, Isaacs FJ, Rinehart J. Nat Biotechnol. 2018 Aug;36(7):638-644. PubMed (Link opens in...

...capacity to give rise to an entire organism. iPSC technology removes the possible ethical concerns related...tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation...Transdifferentiation Plasmids below. Many of the techniques for reprogramming, differentiation, and transdifferentiation... lineage conversion of acinar cells. Nature Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic...oligodendroglial cells by direct lineage conversion. Nat Biotechnol. 2013 May;31(5):434-9. Wernig Fibroblasts Oligodendrocyte...myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol. 2013 May;31(5):426-33. Tesar Fibroblasts Medium...

...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre. ... are determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity.... mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (...for mycoplasma contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages...

...104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David Liu 104070 pTECH-MbAcK3RS...104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial TAG David Liu 104072 pTECH-MbPylRS...M. mazei Cyanobacterial TAG Lital Alfonta 99222 pTECH-chPylRS(IPYE) PylRS Chimeric p-iodo-L-phenylalanine...-L-phenylalanine Bacterial TAG David Liu 104073 pTECH-MmPylRS(IPYE) PylRS M. mazei m-iodo-L-phenylalanine...

... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

...bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini... expressed in vivo . Plasmid ID Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent...Reporter Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium....small molecule, the reporter plasmid can be used to detect the presence of that small molecule and sometimes...) in the plasmid. Other similar reporters for detecting maltose can be found here . pAra Series Various...

...assembled a list of FAQs about using the lab's CRISPR technology....assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong, et. al, 2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow...

...of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...bp from the predicted cleavage site to ensure detection would not be impacted by a small indel at the ...in Molecular Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR...PubMed . Western blotting: sample preparation to detection. Eslami A, Lujan J. J. Vis. Exp . 2010...

...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent...more about pHlash sensor in our 28 Hot Plasmid Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/...investigating regions controlling transcription Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for...investigating regions controlling transcription Pete Stecha Return to Top Expression Constructs Luciferase ...

...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...serotypes AAV1, AAV2, AAV5, AAV8, AAV9, AAVrg, the Caltech systemic serotypes, or others, it will be listed...

...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...collection includes anti-glypicans that accurately detect glypican presence and explore glypican function...that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse or search...

...son of sevenless homolog 2 (Drosophila) FLJ25596 TEC tec protein tyrosine kinase MGC126760, MGC126762, PSCTK4...subfamily 4, group A, member 3 CHN, CSMF, MINOR, NOR1, TEC NR5A1 nuclear receptor subfamily 5, group A, member...potential pathogens each day. Their ability to protect against infection depends on two systems that work... have been infected by a foreign pathogen. They detect infected cells via receptors such as KIR and NKG2D... (C-C motif) ligand 25 Ckb15, MGC150327, SCYA25, TECK CCL26 chemokine (C-C motif) ligand 26 IMAC, MGC126714...

...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample--just to...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

...2009 (Hoshino & Fujii), the locus-specific ChIP technologies enable us to purify specific genomic regions...interacting with those regions. Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation...DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. ...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

...Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4):546, 548, 550. PubMed . Optimization... R, Heinz N, Hampf M, Bujard H, Gossen M. BMC Biotechnol . 2010 Nov 24;10:81. doi: 10.1186/1472-6750-10...-10-81. PubMed . Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions...

... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...

...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ...