...qPCR Using SYBR Green Technology AAV Titration by qPCR Using SYBR Green Technology You may also like......particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. Protocols...genome-containing particles of an AAV prep using SYBR green technology. This protocol was validated using an internal...following protocol in your qPCR instrument using SYBR detection: 98 °C 3 min / 98 °C 15 sec / 58 °C 30 sec / read... A, Baiker A. Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...

...Eye protection and face shields, as needed Guidelines Your lab coat should be buttoned to protect your... personal protective equipment (PPE) for BSL-1 and BSL-2 labs. Protocols... Protocols Personal Protective Equipment...Equipment (PPE) for BSL-1 and BSL-2 Labs Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Intro to...healthy humans. Many of the precautions are meant to protect individuals from potential accidents such as spills...Close-toed shoes and long pants should be worn to protect your legs and feet from potential spills. If wearing...shoes, cloth coverings or “booties” can be used to protect your feet. Pro-Tip If they are able, sometimes ...

...tips for mammalian cell culture Aseptic Technique Aseptic technique is a critical skill for success in numerous... the key strategies of aseptic technique, including personal protective equipment, setting up a clean ...Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once ...preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction... process when requesting samples from Addgene Technology Transfer How to Order A step-by-step walkthrough...

...Video Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when...Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens...questions. Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. ...Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your...

...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction... to an open UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from ...the desired DNA fragment from the gel. Notes: To protect the UV box, it is a good idea to place the gel ...will not significantly reduce the UV, but will protect the UV box from being cut by the razor blade. Try...

...between different types of hazards and to provide protective measures for each level, as each level has different...Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps... entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you...re doing your work in the safest way possible. Protecting yourself and working smart in the lab is important...

...Introduction Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe...µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution... the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells ...

...Western blot is a technique used to separate proteins by size followed by detection using antibodies specific...gel imager with chemiluminescence detection or a dark room to detect the bands. Tips and Troubleshooting...

...Bacterial Transformation Summary The following technique can be used to easily move any piece of DNA from... by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss...from one plasmid to another. However, the same technique can be used to move promoters, selectable markers...

...for a stock mixture is an essential laboratory technique, as imprecise measurements can affect the outcome...weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials...

...insert and vector backbone are known and thus this technique can be quickly used to verify your plasmid. The... should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting...

...Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the biosafety...higher speeds and are used for more specialized techniques. Regardless of the type of centrifuge you use...

...ET SSB) to the isothermal reaction mix. ET SSB protects 3’ ssDNA ends from the ssDNA-specific endonuclease... is to “stitch” together several oligos. This technique is especially useful for introducing promoters...

...advantage of the physical properties of light to detect small objects. Two of the most important properties...Procedure Set up your microscope by removing any protective cover that may be on it and plugging in the power...

...EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this chemical. Note... damage to the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield...

...the literature for an appropriate sterilization technique if you are working with any weird and wonderful... into your ~60 ℃ molten gel mix using sterile technique. Swirl the agar bottle to ensure even distribution...

...preparing and handling lentiviral particles. Personal protective clothing should be worn at all times. Use plastic...gene delivery in vivo. Zufferey R et. al. Nat Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating...

...do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start by streaking...

...MCS of an empty vector. However, the following technique can be used just as easily to add short tags or...

...digestion is commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also...