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Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only
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pDONR223-TEC
Plasmid#23885DepositorInsertTEC (TEC Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TEC-1/1
Plasmid#91252PurposeProtein expression and purification of human SH3 domain construct TEC-1/1DepositorInsertTEC-1/1 (TEC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTec_mCherry
Plasmid#229145PurposePromoter strength evaluation of PtecDepositorInsertmCherry
UseTagsExpressionBacterialMutationNoPromoterPtecAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTEC15
Plasmid#30174DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsWasabiExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC27
Plasmid#30182DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagstdTomatoExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC19
Plasmid#30178DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsCrimsonExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC18
Plasmid#30177DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsEBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC22
Plasmid#30181DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagstdKatuschka2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC16
Plasmid#30175DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsmOrange2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTEC17
Plasmid#30176DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsTealExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC20
Plasmid#30179DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsmKate2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEC21
Plasmid#30180DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsKaedeExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTEcoliDHFR_R166QTS
Plasmid#91242Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationR166QTSPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-B
Plasmid#58307PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-nTVMVPteZtecTVMVP
Plasmid#116082PurposeExpresses an engineered TVMV proteaseDepositorInsertnTVMVPteZtecTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-BrainbowTEC
Plasmid#82405PurposeGateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HADepositorInsertstdTomatoMYC fusion
EGFP
E2CrimsonHA
UseTagsHA and MYCExpressionMutationPromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH474_pTech_Para-supP
Plasmid#163718PurposeArabinose-inducible expression of supPDepositorInsertsupP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-nTVMVPteZtecTVMVPcx
Plasmid#116087PurposeExpresses an engineered TVMV proteaseDepositorInsertnTVMVPteZtecTVMVPcx
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only