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Addgene

pSEVA12TEC-I
(Plasmid #58308)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58308 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    SEVA
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    Growth may be sluggish due to high expression levels of genomic cassette.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Genomic cassette
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Zeta/Epsilon. Toxin-Antitoxin parts. Part source: Dr. Urszula Zielenkiewicz (Dept of Microbial Biochemistry, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's NGS found a 1209bp transposase insertion between the RepA and Epsilon Antitoxin sequence. The effect of this insertion is unknown. If you are interested in the version without the insertion, please contact the depositing lab (Tom Ellis lab).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA12TEC-I was a gift from Tom Ellis (Addgene plasmid # 58308 ; http://n2t.net/addgene:58308 ; RRID:Addgene_58308)
  • For your References section:

    GeneGuard: a Modular Plasmid System Designed for Biosafety. Wright O, Delmans M, Stan GB, Ellis T. ACS Synth Biol. 2014 May 13. 10.1021/sb500234s PubMed 24847673