...several washes, this tag can be cleaved by the bdNEDP1 protease, thus releasing purified nanobodies from the...common protein purification methods. Read on to learn more about nanobodies and how their structure and...primary antibodies raised in different species, or at least from different IgG subclasses, so that secondary... and the target antigen to about 2 nm, which can lead to higher resolution for super-resolution microscopy...rabbit nanobodies. If you’re interested, you can learn more about how nanobodies are identified in this...University Use Nanobodies to Control Protein Activity Learn more about protein tags Read about E. coli strains...

...journals. In addition, it is my pleasure to interact with my colleagues in meetings of scientific societies..."Biology is Method", i.e. new technologies often lead to big discoveries in biology. Therefore, my lab...translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004. In addition,...much enjoyed interactions with many excellent colleagues at NYU and visiting scientists, such as seminar..., I've been very lucky to work with excellent colleagues in my lab. On the other hand, I feel that researchers... conventional research. So, I hope my younger colleagues may be able to develop new technologies, hopefully...

...the progress of the project. After that rather unpleasant experience I thought: I could do this job so ...and disadvantages of this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career...Technical Correspondent or similar. While there isn’t a clear distinction between these job titles, they may be...particularly tricky cases, team discussions can often lead to otherwise unobtainable solutions. To some extent...support can add a variety of skills to your CV and lead to other job opportunities (though it is not uncommon...Occasionally it can also be necessary to take the lead and be assertive. You develop an understanding of... Now? Academic v. Non-Academic Career Decisions Learn about Science Career Options Explore Tips for Technical...

...define your scope from the onset. Perhaps you, a colleague, or your adviser was invited to write on a particular...to get a sense of the themes in the field and to learn what people cared about. I also used reviews to ...beginning I felt like this was cheating. Eventually I learned that it’s practical because there simply isn’t ...enough time to read every single paper. I also learned to appreciate the discussion sections of the primary... down and get anything done, so unless I had at least a two hour block, I wouldn’t work on the review....free two hour block of time was. Eventually, I learned that I was most productive in short bursts (even... could find the original paper if you wanted to learn more. Another example: we made a schematic of all...

... science trainees still have to lead on their own development. Please see the AMA itself for all sorts... a great way to establish yourself as a thought leader and have real conversations with other scientists...forums and try to keep discussions on-topic and, at least sometimes, civil. r/Science in particular hosts ...answered many questions in the AMA but, as you’ll learn below, even with many resources at her disposal,...potentially hostile posters (this advice included leaving out the term “implicit bias” which is apparently...academic one for the majority of scientists and I am pleased to see graduate and postdoc programs beginning ...

...Previous Post on CRISPR Mouse Models Learn about Homology Directed Repair Learn about Cas9 Ribonucleoprotein ...previous methods yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er...re fortunate that there’s a guide that directs cleavage right before the stop codon!   Design your repair...Miura et al. (2018) recommend targeting sites at least 100 bp away from the target exon on either side....in a two-guide floxed experiment, if one guide cleaves less efficiently than the other, a partial insertion...situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018) (1):25. DOI: 10.1186/s13059...

...High School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies...that it’s never too early to start networking! Learning about careers at Addgene Afterwards, the students...: “Kory was awesome!”. The students also got to learn about intellectual property from our Tech Transfer...career paths, Michelle Healy said “You are always learning and that means it is possible to switch your field...Additional Resources Visit the MassBioEd Website Learn more about Dennis-Yarmouth Regional High School ... Blog Posts Get Career Advice from Our Bloggers Learn How to Improve Your Networking Skills  ...

...You wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn about other...difference? Individual changes to our behavior can lead to cultural shifts that can have bigger impacts....even one less run a week makes a big difference. Clean out freezers so you can make room and don’t have...

...nonimmigrants). The foreign worker must possess at least a bachelor's degree or its equivalent and state ...books, journals online and/or in print. Having a lead role or critical role at a prestigious institution...successes in the performing arts- not relevant at all. Please do not include the fact that you are a classically...company you must show that your company employs at least 3 full-time people engaged in researcher activities...from the point of view of experts in your field. Please remember, this is not a job reference letter. This...awards, presentations, in .pdf format.  Last but not least, NETWORK! Networking is extremely important for ...business development, finance, and operations. To learn more, visit www.propelcareers.com. Casseus Law: ...

...perhaps there is a clear winner, is there something that we can invest our time into learning and extract the...timers: You need to learn the language and what the parameters represent. The best learn-by-doing tool is...for improvement and a year later it became quite clear that others thought the same. Since January 2013...microhomology aware”. Trends: Fewer tools are being released with advanced functionality, it seems these kinds...blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu Intérieur project,..."DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed...

...fluorescence. In Brainbow 2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize ...mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic...Cre undergoes exactly one recombination event, leading to each copy of the transgene expressing either... tags instead of XFP to optimize it for tissue clearing, a method that makes tissues, such as the brain...visible. Chemical tags are more resistant to tissue clearing methods and can be labeled by synthetic fluorophores...blog Read an introduction to live cell imaging Learn more about the origin of the Brainbow system Visit...Browse Addgene’s fluorescent protein collection Learn about fluorescent tools for in vivo imaging ...

...this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab...have the best time management skills, but I’ve learned a lot since my days as an undergrad working with... variety of projects. I was therefore forced to learn a variety of ways to manage my time. As a busy researcher...distractions: Probably the easiest way to do this is to leave your phone in your bag on “Do Not Disturb” during... recommend the book, “Bored and Brilliant” for learning more fantastic reasons to remove yourself from...you have any other time management suggestions, please be sure to note them in the comments! Additional...Get Advice on Building a Grad Student Community Learn about Careers in Science Communication Resources...

...Scientists enjoy learning new things. Building new relationships is all about learning new things from ...scientists-in-training rarely get the opportunity to learn about the myriad of diverse, nonacademic careers...worth!) with academic scientists who can’t help us learn about the nonacademic sphere where most of us will...really, always). Informational Interviews are for learning and for expanding your web of connections. A mistake... are always setting up your next opportunity to learn. Additional Reading More "Career Advice for Scientists...

...effect reprogramming rates and such a number maybe misleading. Integrating iPSC delivery methods Overall, integrating...incomplete silencing of reprogramming transgenes, which leads to incomplete reprogramming. Additionally, lingering...suppressor genes and/or oncogenes, potentially leading to the presentation of a cancer-like phenotype ...regulating transgene expression. Check out Sano et al to learn how microRNAs can be used to regulate Sendai virus... don’t integrate or replicate. Their small size leads to higher transfection efficiencies and they tend...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...all applications of iPSCs, but there should be at least one method that can help you with your research ...

...team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate...their behavior. These ants become “zombies” and leave their foraging trail. They climb upwards until they... The ants bites down and locks their jaws into a leaf, and dies as its muscles atrophy. After the ant’...ant’s body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...adult worms develop in the digestive tract and release eggs in the bird feces, which get eaten by another...water. There, the worm emerges to find a mate, leaving the insect dead or dying in the water. It’s thought...

...targeting integrins, will be released early this year.  In September, we released the fourth edition of our...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...services available in South Korea.  In addition, we released the MORF collection, a comprehensive transcription... our export license application service, we’re pleased to announce that we are now able to ship rabies...

...their research leading up to the discovery of the mechanism of dual-RNA-guided DNA cleavage in adaptive ...crRNA could be combined into one RNA to direct cleavage the result was a simpler 1 protein - 1 RNA system...cells but this can’t be used in people until we learn more about safety and more about the how to use ...cool biology can be and how much we have yet to learn. As Dr. Mello said, “Cells have been in the information...sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR...

...England Biolabs and Labconscious. I was eager to learn about practices I could bring both to Addgene, and...impact as scientists. Many scientists are already leading the cultural change toward responsibility in our...polystyrene will likely end up in a landfill, but at least we can reduce the rate at which that happens and... it offsets the cost of regular waste services. Learn about how to start a glove recycling program at ...on culture. Encourage your workplace to become a leader in sustainability. Sign up for blog posts and...companies, and universities to be sustainability leaders. Take initiative to bring ideas to your workplace...

... sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...SunTag plasmids have coveted “blue flames,” it’s clear that the system is broadly applicable across many...eBook Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

...exploration. Here, we delve into the importance of leaving room for serendipity during your PhD studies and...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative solutions...discoveries. So, on your academic journey, remember to leave a little space for the unexpected — it might just...Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned to industry...Experimenting with New Careers While in Grad School How to Lead a Great Meeting Form Your Own Peer Mentoring Group...