We narrowed to 390 results for: promoter
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TypeBlog Post...Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...
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The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post... and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity... -
Pushing the Limits of DNA Assembly
TypeBlog Post...assembly reaction. DNA fragments with the desired promoter, selectable marker, and target gene, for example... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...antibiotic resistance genes, selectable markers, promoters, origins, tags, and certain ORFs. Their sophisticated... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...studying how that one protein interacts with that one promoter can tell you, it can be easy to get lost in the... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...characterize the function of certain regions in a promoter or gene, as well as to study the effects of inactivating... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...and tech transfer communities who help guide and promote Addgene in its mission to make research easier.... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post... and offer congratulations on job changes and promotions. Follow academic scientists on ResearchGate ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...plasmid with a promoter that will be functional in your host organism. Host Relevant Promoters Representative...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated ...proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently...T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more... expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from...Gileadi lab Worm unc-54, variety of worm gene promoters C. elegans vector kit - Collection... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable ...Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
Plant Plasmids and Resources
TypeCollection...containing promoters that drive general and species-specific gene expression (see the column ‘promoter’ in the...for, such as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI..., such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to ... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots - Vladimir... (Link opens in a new window) - The initiative promotes interdisciplinary exchange, open technologies,... -
Adenovirus Plasmids
TypeCollection...of viruses containing transgene under a chosen promoter Vogelstein 16403 pShuttle-CMV Shuttle For production...production of viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle For production...GFP-trackable viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production...GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle... transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP Shuttle For production...viruses containing transgene under mouse cofilin promoter (MCP) Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus... -
TALEN Plasmids and Kits
TypeCollection...DNA sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into...the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations ...directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro...galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built...for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN expression...double-transfected mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs through...expression. The vectors listed below have the EF1α promoter for efficient mammalian cell expression, and encode... -
CRISPR Plasmids - Drosophila
TypeCollection...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants...-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused...edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select...variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed... -
CRISPR Plasmids - Plants
TypeCollection...opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9...edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression... -
CRISPR Plasmids - Base Edit
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable ...PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
The Pleiades Promoter Project
TypeCollection...Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (Link opens...Positive Control CAG promoter pEMS1293 cre CAG promoter pEMS1164 EGFP/cre CAG promoter pEMS1114 EGFP/cre/...cre/NLS CAG promoter pEMS1157 EGFP/NLS CAG promoter pEMS1277 EGFP/NLS CAG promoter pEMS1294 intron-lacZ/...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression...mouse brain through these human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below...intron-lacZ/NLS CAG promoter pEMS1488 intron-lacZ/NLS Ple2 ADORA2A pEMS1142 EGFP/NLS Ple3 ADORA2A pEMS1143 EGFP/...Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Negative... -
CRISPR Plasmids - Prime Edit
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...released into the environment. Various factors promote natural transformation in different bacteria such...