Optogenetics + CRISPR, Using Light to Control Genome Editing
Type
Blog Post
...reversibility of the genome editing process. Read on to learn about the various light-controlled CRISPR tools ... Sato lab unveiled a photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015)...system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9 into N-terminal ... moieties move apart, allowing DNA binding and cleavage. This technique was applied to create photoswitchable...inhibited by the unfolding of LOV, allowing the release of Cas9. A noteworthy application of the CASANOVA...in U2OS cells. Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later...PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation of amino ...