-
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP
Plasmid#40640DepositorInsertNICD3 (NOTCH3 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
LYN11-FRB-CFP
Plasmid#38003DepositorUseTagsECFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-cMYC
Plasmid#20324DepositorInsertcMYC (Myc Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-GASC1
Plasmid#24214PurposeMammalian expression of human JMJD2C with HA tagDepositorInsertGASC1 (KDM4C Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorInsertc-Myc (Myc Mouse)
UseTagsExpressionBacterialMutationNucleotides 2578-3294PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt3
Plasmid#66832PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt3 (ESYT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
LYN11-FRB-mcherry
Plasmid#38004DepositorUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVAX1-BMP7
Plasmid#137910PurposeConstitutive mammalian overexpression vector for human bone morphogenetic protein 7 cDNADepositorInsertBMP7 (BMP7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 (FL)
Plasmid#24388DepositorInsertBeclin1 (BECN1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK_Gaussia_luciferase
Plasmid#154459PurposeExpression of gaussia luciferase and firefly luciferaseDepositorInsertGaussia luciferase
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW
Plasmid#75469PurposeFlp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertFlp-DOG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-humanTRIM5-FOS
Plasmid#79066PurposeExpress human TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(human)-FOS (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CHMP2A_GFP_N_term
Plasmid#31805DepositorInsertCHMP2A (CHMP2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1
Plasmid#13798DepositorInsertJnk1a1 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-FYN-WT
Plasmid#74509PurposeOverexpression of FYN in mammalian cellsDepositorInsertFYN (FYN Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only