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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 38003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-N1
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Backbone manufacturerCLONTECH
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5052
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameLYN
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SpeciesH. sapiens (human)
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Insert Size (bp)33
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GenBank IDNM_002350.3
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Entrez GeneLYN (a.k.a. JTK8, SAIDV, p53Lyn, p56Lyn)
Gene/Insert 2
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Gene/Insert nameMTOR
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Insert Size (bp)276
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GenBank IDNM_004958.3
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
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Tag
/ Fusion Protein
- ECFP (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTamas Balla
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LYN11-FRB-CFP was a gift from Robin Irvine (Addgene plasmid # 38003 ; http://n2t.net/addgene:38003 ; RRID:Addgene_38003) -
For your References section:
PI4P and PI(4,5)P2 Are Essential But Independent Lipid Determinants of Membrane Identity. Hammond GR, Fischer MJ, Anderson KE, Holdich J, Koteci A, Balla T, Irvine RF. Science. 2012 Jun 21. 10.1126/science.1222483 PubMed 22722250