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Plasmid#202513PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_SigP:NLuc
Plasmid#197263PurposeCloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shIrak1-2
Plasmid#180398PurposeProducing AAV that encodes mouse Irak1 shRNA-2 with miR-E backboneDepositorInsertshIrak1-2 (Irak1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shIrak1-3
Plasmid#180399PurposeProducing AAV that encodes mouse Irak1 shRNA-3 with miR-E backboneDepositorInsertshIrak1-3 (Irak1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shIrak1-1
Plasmid#180397PurposeProducing AAV that encodes mouse Irak1 shRNA-1 with miR-E backboneDepositorInsertshIrak1-1 (Irak1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE30-VRN1-R249E-R289E-R296E
Plasmid#159532PurposeExpresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coliDepositorInsertVERNALIZATION1 (VRN1 Mustard Weed)
UseTagsSix-Histidine tagExpressionBacterialMutationThree charge reversal mutations, namely R249E R28…PromoterT5Available sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1sgRNA2
Plasmid#160946PurposeGuide RNA 2 to generate Nr4a1 knockout by CRISPRDepositorInsertNr4a1 gRNA 2 (Nr4a1 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-ARF6-T157N-mNeonGreen
Plasmid#162028PurposeFast cycling ARF mutantDepositorInsertARF6 (ARF6 Human)
UseLentiviralTagsExpressionMutationT151NPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-ARF5-T161A-mNeonGreen
Plasmid#162026PurposeFast cycling ARF mutantDepositorInsertARF5 (ARF5 Human)
UseLentiviralTagsExpressionMutationT161APromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P4-P1R:PER36modp
Plasmid#128431PurposeGateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).DepositorInsertPEROXISADE36 (AT3G50990 Mustard Weed)
UseGateway promoter entry cloneTagsExpressionMutationPremature stop codons introduced into the Xero1 (…PromoterPEROXISADE36Available sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDF1-MCS2-EF1-Puro-RGS7-E383K
Plasmid#113731PurposeExpress mutant RGS7- E383K- in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationE383KPromoterCMVAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-MCS2-EF1-Puro-RGS7-R44C
Plasmid#113729PurposeExpress mutant RGS7- R44C- in mamalian cellsDepositorInsertRGS7 (RGS7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT2-HA-RAPGEF2(S1244A/S1248A)
Plasmid#110163PurposeExpression of human RAPGEF2 (S1244A/S1248A) in mammalian cellsDepositorInsertRAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
UseTagsHAExpressionMammalianMutationS1244A/S1248APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/181/666E
Plasmid#105314PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/181/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-646
Plasmid#92289PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cellsDepositorInsertdGW182 CED 7W7A (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215 with the following point …PromoterAc5Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-sim1
Plasmid#67908PurposeInducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.DepositorInsertPIAL2 (AT5G41580 Mustard Weed)
UseTagsMBPExpressionBacterialMutationE281-A496; VEDL 425-428 AAAPromoterP-lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Keratin-17
Plasmid#56164PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659DepositorInsertKeratin (KRT18 Human)
UseTagsmCardinalExpressionMammalianMutationV282LPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV6-Entry IL36RN R48W
Plasmid#58324Purposeentry vector for human IL36RN R48W mutantDepositorInsertIL36RN (IL36RN Human)
UseEntry vectorTagsmyc-DDKExpressionMutationR48W: c.142C>T, p.Arg48TrpPromoterCMVAvailable sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-Entry IL36RN P76L
Plasmid#58322Purposeentry vector for human IL36RN P76L mutantDepositorInsertIL36RN (IL36RN Human)
UseEntry vectorTagsmyc-DDKExpressionMutationP76L: c.227C>T,p.Pro76LeuPromoterCMVAvailable sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-Entry IL36RN Κ35R
Plasmid#58320Purposeentry vector for human IL36RN K35R mutantDepositorInsertIL36RN (IL36RN Human)
UseEntry vectorTagsmyc-DDKExpressionMutationΚ35R: c.104A>G, p.Lys35ArgPromoterCMVAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only