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Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-mCherry-CyrCR1
Plasmid#128768PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.DepositorInsertCyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-Plus-mCherry
Plasmid#191231PurposeTo express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertAsCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationE174R, R951K, R955APromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK7WGF2::hCas9
Plasmid#46965PurposeExpesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPExpressionMutationPromoter35SAvailable sinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R1
Plasmid#61382PurposeExpresses HA tagged TRAIL receptor 1DepositorInsertTRAIL receptor 1
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R1 leader peptide (aa1-23) replaced with TN…PromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-DsRed-cNLS
Plasmid#138528PurposeInducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tmem214 pmRFP-N2 (1348)
Plasmid#62047Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem214 (TMEM214 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-InPAkt
Plasmid#181914PurposeIndicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertInPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hRec8(297-506)-mNeonGreen
Plasmid#174717PurposeStable expression of fluorescent Separase cleavage sensor with human Rec8DepositorInsertRec8 (REC8 Human)
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation297-506PromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN42
Plasmid#61345PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS10 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)
Plasmid#86414Purposenuclear split GFP11 reporter without mCherryDepositorInsertNOP1pr-GFP11-PUS1
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1prAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1553 CMV-EBNA1 (delta GGA)
Plasmid#26648DepositorInsertEpstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)
UseTagsExpressionMammalianMutationdeletion of 224 amino acids of EBNA-1 (aa101-324 …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only