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Plasmid#145646PurposeBacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046770
Plasmid#145594PurposeBacterial Expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046797
Plasmid#145676PurposeBacterial Expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046878
Plasmid#145743PurposeBacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-P03
Plasmid#120014PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
VCDstub-GFP
Plasmid#33122DepositorInsertPcdh-γA3 (Pcdhga3 Mouse)
UseTagsEGFPExpressionMammalianMutationContains a signal sequence fused to a very small …PromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
ΔCT190-GFP
Plasmid#33118DepositorInsertPcdh-γA3 (Pcdhga3 Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion of the last 190 aa of the variable cytop…PromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTarget-Dual
Plasmid#207884PurposeCarries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.DepositorInsertsI-OnuI target site
I-GpeMI target site
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
M-NylC_pET28a
Plasmid#215409PurposeExpression construct for M-NylC gene, codon optimized for expression in E. coliDepositorInsertM-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1161
Plasmid#217964PurposeFor RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFP, APEXExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ZmAFB2/3 b2
Plasmid#188425PurposeZea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2DepositorInsertAuxin Signaling Receptor AFB2/3 b2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ZmAFB2/3 a
Plasmid#188423PurposeZea Mays Nuclear Auxin Signaling Receptor AFB2/3 ADepositorInsertAuxin Signaling Receptor AFB2/3 a
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP4G-REL2N91-ccdB
Plasmid#188444PurposeZea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDESTDepositorInsertREL2N91/ccdB
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ZmIAA8ΔPB1
Plasmid#188432PurposeZea Mays Nuclear Auxin Signaling Repressor IAA8DepositorInsertAuxin Signaling Repressor IAA8deltaPB1
UseCloningTagsExpressionMutationdeleted PB1 domainPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB441
Plasmid#185131PurposeSwi6 full length with L->A mutated in nuclear localization signalDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSWI6 deletion of amino acids 450-458PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppwd1 (PPWD1 Human)
UseTags(His)6-thrombinExpressionBacterialMutation473-646-W601APromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB394
Plasmid#185086PurposeNMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNma111 KKR 9-11 AAA; KRK 28-30 AAAPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c014
Plasmid#175451PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c011
Plasmid#175454PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c011
Plasmid#175449PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.DepositorInsertCD44 (CD44 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only