Addgene

Showing: 1721 - 1740 of 15028 results

Results per page:

Sort results by:

pAcGFP-MALAT1M

Plasmid

#120382

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

MALAT1 (MALAT1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-TSC2

Plasmid

#192440

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2 (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-NLS-copGFP-EF1-BlastiS

Plasmid

#132772

Purpose

Spills into the cytoplasm during nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

NLS-copGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Use

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSQT834

Plasmid

#53371

Purpose

Csy4 and Cas9 nuclease expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Csy4-T2A-Cas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Kunihiko Naito, Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NLS-PhoCl-mCherry

Plasmid

#87691

Purpose

Express NLS-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NLS-PhoCl-mCherry

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Use

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
M-NMn-VP64

Plasmid

#48676

Purpose

Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Expression

Mutation

nuclease-null

Promoter

CMV

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
NET47 pEGFP-N2 (593)

Plasmid

#61986

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET47 (TM7SF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
M-ST1n-VP64

Plasmid

#48675

Purpose

Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Expression

Mutation

nuclease-null (D9A D598A H599A N622A)

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-H2GH

Plasmid

#182202

Purpose

Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

histone2B-HaloTag-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNuc-cis

Plasmid

#202796

Purpose

RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

Depositor

David Edgell

Article

Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

Insert

TevSpCas9, sgRNA cassette, oriT, CenArsHis

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only
pGL3-mArg1 base promoter -31/-2365

Plasmid

#34570

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 base promoter -31/-2365 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Mutation

Promoter

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Use

Tags

Strep II-MBP

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas1_Cas2/3

Plasmid

#89240

Purpose

Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas1 and Cas2/3

Use

Tags

Strep II tag on Cas1

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP-Jpx9C104T

Plasmid

#120381

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

C321T

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
M-SPn-VP64

Plasmid

#48674

Purpose

Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Expression

Mutation

nuclease-null

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
NKP64 GFP (1007)

Plasmid

#62020

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

SLC38A10 (SLC38A10 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only