pGL3-mArg1 base promoter -31/-2365
(Plasmid
#34570)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsMouse Arg1 reporter constructs cloned into the pGL3 backbone are UNSTABLE when grown at 37 degrees for large-scale preps. We recommend that plasmids be re-transformed and grown overnight at 30 degrees on Amp plates. The next day, scrape the entire plate into 100mL LB-Amp media and grow at 30 degrees (or room temp) for 4-6hr then perform conventional plasmid preps.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArg1 base promoter -31/-2365
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SpeciesM. musculus (mouse)
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Insert Size (bp)2334
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Entrez GeneArg1 (a.k.a. AI, Arg-1, PGIF)
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Tag
/ Fusion Protein
- Firefly Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-mArg1 base promoter -31/-2365 was a gift from Peter Murray (Addgene plasmid # 34570 ; http://n2t.net/addgene:34570 ; RRID:Addgene_34570) -
For your References section:
Enhancer-mediated control of macrophage-specific arginase I expression. Pauleau AL, Rutschman R, Lang R, Pernis A, Watowich SS, Murray PJ. J Immunol. 2004 Jun 15;172(12):7565-73. PubMed 15187136