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Plasmid#87429PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1DepositorInsertTJP1 Homology Arms with mEGFP-linker (TJP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-eDHFR-MCS
Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorInsertEGFP-eDHFR-20aa (folA E. coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSSRB274_pAC8-eGFP-hsCRBN
Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot3
Plasmid#37372DepositorInsertHsNot3 (CNOT3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-sfCherry11x4-ß-actin
Plasmid#70220PurposeExpresses sfCherry11x4-ß-actin in mammalian cellsDepositorInsertsfCherry11x4-actin (ACTB Human)
UseTagssfCherry11x4ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab33bb in pCSDEST2
Plasmid#140882PurposeExpression of zebrafish GFP-tagged Rab33bb in mammalian cells.DepositorInsertRab33bb
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-eGFP
Plasmid#122033PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expressionDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Venus-Puma-pEGFP-C1
Plasmid#166739PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, PumaDepositorInsertPuma (BBC3 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-Halo,IRES-eGFP
Plasmid#164519PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
NPC1 His6 EGFP
Plasmid#53521DepositorInsertNPC1
UseTagsEGFP and His6ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-P4Mx1
Plasmid#108121PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-SHP2WT
Plasmid#12283DepositorInsertSHP-2WT (PTPN11 Human)
UseTagsMyc (not canonical: KLISEEDL)ExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSP4-E228Q
Plasmid#80351PurposeExpression of GFP-tagged, dominant negative VPS4 mutantDepositorInsertVPS4-E228Q (VPS4A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-pEGFP-C1
Plasmid#177408PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.DepositorInsertBcl-XL
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-DD-pEGFP-C1
Plasmid#177429PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationThreonine 33 and Serine 35 replaced with aspartic…PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAGGS-EGFP
Plasmid#22212PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertCAGGS-eGFP
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsPatL1_I
Plasmid#146547PurposeMammalian Expression of HsPatL1DepositorInsertHsPatL1 (PATL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertLBR (LBR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mSA-EGFP-TM
Plasmid#39863DepositorInsertmonomeric streptavidin, EGFP, TM helix
UseTagsEGFP, FLAG, and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only