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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES2-EGFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSHP-2WT
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Alt namePTP2c
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Alt namePTP-1D
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SpeciesH. sapiens (human)
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Insert Size (bp)1900
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GenBank IDNP_002825
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Entrez GenePTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
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Tag
/ Fusion Protein
- Myc (not canonical: KLISEEDL) (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byNeel.BG Department of Medicine, Beth Israel Deaconess Medical Center, Boston,Massachusetts 02115, USA.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES2-EGFP-SHP2WT was a gift from Anton Bennett (Addgene plasmid # 12283 ; http://n2t.net/addgene:12283 ; RRID:Addgene_12283) -
For your References section:
The major vault protein is a novel substrate for the tyrosine phosphatase SHP-2 and scaffold protein in epidermal growth factor signaling. Kolli S, Zito CI, Mossink MH, Wiemer EA, Bennett AM. J Biol Chem. 2004 Jul 9. 279(28):29374-85. 10.1074/jbc.M313955200 PubMed 15133037