The C-terminal alpha-alpha superhelix of Pat is required for mRNA decapping in metazoa.
Braun JE, Tritschler F, Haas G, Igreja C, Truffault V, Weichenrieder O, Izaurralde E
EMBO J. 2010 Jul 21. 29(14):2368-80. PubMed Article
Braun JE, Tritschler F, Haas G, Igreja C, Truffault V, Weichenrieder O, Izaurralde E
EMBO J. 2010 Jul 21. 29(14):2368-80. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146544 | pLNHA-C1-HsPatL1_I | Mammalian Expression of HsPatL1 |
| 146545 | pLNHA-C1-HsPatL1-mut1_I | Mammalian Expression of HsPatL1-mut1 |
| 146546 | pLNHA-C1-HsPatL1-mut2_I | Mammalian Expression of HsPatL1-mut2 |
| 146547 | pT7-EGFP-C1-HsPatL1_I | Mammalian Expression of HsPatL1 |
| 146548 | pT7-EGFP-C1-HsPatL1-mut1_I | Mammalian Expression of HsPatL1-mut1 |
| 146549 | pT7-EGFP-C1-HsPatL1-mut2_I | Mammalian Expression of HsPatL1-mut2 |
| 146658 | pLNHA-C1-HsLSm1_J | Mammalian Expression of HsLSm1 |
| 146659 | pLNHA-C1-HsPatL1_1-398_J | Mammalian Expression of HsPatL1_1-398 |
| 146660 | pLNHA-C1-HsPatL1_1-517_J | Mammalian Expression of HsPatL1_1-517 |
| 146661 | pLNHA-C1-HsPatL1_70-513_J | Mammalian Expression of HsPatL1_70-513 |
| 146662 | pLNHA-C1-HsPatL1_70-770_J | Mammalian Expression of HsPatL1_70-770 |
| 146663 | pLNHA-C1-HsPatL1_70-770del399-516_J | Mammalian Expression of HsPatL1_70-770del399-516 |
| 146664 | pLNHA-C1-HsPatL1_353-770_J | Mammalian Expression of HsPatL1_353-770 |
| 146665 | pLNHA-C1-HsPatL1_399-516_J | Mammalian Expression of HsPatL1_399-516 |
| 146666 | pLNHA-C1-HsPatL1_399-770_J | Mammalian Expression of HsPatL1_399-770 |
| 146667 | pLNHA-C1-HsPatL1_517-770_J | Mammalian Expression of HsPatL1_517-770 |
| 146668 | pLNHA-C1-HsPatL1-del399-516_J | Mammalian Expression of HsPatL1-del399-516 |
| 146669 | pLNHA-C1-HsPatL1-mut3_J | Mammalian Expression of HsPatL1-mut3 |
| 146670 | pRSFDuetP-HsPatL1_517-767_J | Bacterial Expression of HsPatL1_517-767 |
| 146671 | pRSFDuetP-HsPatL1_517-767del664-673_J | Bacterial Expression of HsPatL1_517-767-del664-673 |
| 146672 | pRSFDuetP-HsPatL1_517-770-Mut1_J | Bacterial Expression of HsPatL1_517-770-Mut1 |
| 146673 | pRSFDuetP-HsPatL1_517-770-Mut2_J | Bacterial Expression of HsPatL1_517-770-Mut2 |
| 146674 | pRSFDuetP-HsPatL1_517-770-Mut3_J | Bacterial Expression of HsPatL1_517-770-Mut3 |
| 146675 | pRSFDuetP-HsPatL1_517-770-R591AR595AK625AK626A_J | Bacterial Expression of HsPatL1_517-770-R591AR595AK625AK626A |
| 146676 | pT7-EGFP-C1-HsLSm1_J | Mammalian Expression of HsLSm1 |
| 146677 | pT7-EGFP-C1-HsPatL1_1-69_J | Mammalian Expression of HsPatL1_1-69 |
| 146678 | pT7-EGFP-C1-HsPatL1_1-398_J | Mammalian Expression of HsPatL1_1-398 |
| 146679 | pT7-EGFP-C1-HsPatL1_1-517_J | Mammalian Expression of HsPatL1_1-517 |
| 146680 | pT7-EGFP-C1-HsPatL1_70-516_J | Mammalian Expression of HsPatL1_70-516 |
| 146681 | pT7-EGFP-C1-HsPatL1_70-770_J | Mammalian Expression of HsPatL1_70-770 |
| 146682 | pT7-EGFP-C1-HsPatL1_70-770del399-516_J | Mammalian Expression of HsPatL1_70-770del399-516 |
| 146683 | pT7-EGFP-C1-HsPatL1_353-770_J | Mammalian Expression of HsPatL1_353-770 |
| 146684 | pT7-EGFP-C1-HsPatL1_399-770_J | Mammalian Expression of HsPatL1_399-770 |
| 146685 | pT7-EGFP-C1-HsPatL1_518-770_J | Mammalian Expression of HsPatL1_518-770 |
| 146686 | pT7-EGFP-C1-HsPatL1-del399-516_J | Mammalian Expression of HsPatL1-del399-516 |
| 146687 | pT7-EGFP-C1-HsPatL1-mut3_J | Mammalian Expression of HsPatL1-mut3 |
| 147455 | pT7-EGFP-C1-HsEDC4_S | Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter. |