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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1tight

Plasmid

#89362

Purpose

A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1tight (MAP2K1 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Human, Synthetic)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL8B-GFP

Plasmid

#67404

Purpose

Mammalian expression of hArl8b with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Use

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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HA-GABARAPL1

Plasmid

#137759

Purpose

Expression of GABARAPL1

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

Nora Heisterkamp, John Groffen

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
HA-GABARAPL2

Plasmid

#137761

Purpose

Expression of GABARAPL2

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-Flag MKK6(glu)

Plasmid

#13518

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(glu) (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-207Glu Thr-211Glu

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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myc-Atg14

Plasmid

#86747

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Atg14 (ATG14 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-K179M

Plasmid

#73409

Purpose

Expresses kinase-inactive mutant of Akt with HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

changed K179M to make Kinase inactive mutant

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABEpuro-HA-MEK1

Plasmid

#53195

Purpose

Expresses HA tagged human MEK1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-ARL8A-GFP

Plasmid

#67403

Purpose

Mammalian expression of hArl8 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
901 pLNCX myr HA Akt1

Plasmid

#9005

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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Dox-Akt

Plasmid

#207328

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

rac protein kinase-alpha (AKT1 Human)

Use

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 129 - 480

Promoter

Tet Response Element

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1(apf)

Plasmid

#13846

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(apf) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3A

Plasmid

#137756

Purpose

Expression of LC3A

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3A (MAP1LC3A Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-PRAS40 WT

Plasmid

#86758

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge