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Plasmid#125137PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).DepositorInsertmNeon/mScarletP2A Rac1-N17
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 17th AA in Rac1 ch…PromoterCAGAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)
Plasmid#65524PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
JL202: pMVP/mCherry-DEST
Plasmid#121853PurposepMVP destination vector, empty expression vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TF-bio-His
Plasmid#52184PurposeExpresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTF (TF Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
B. fragilis 16S toehold switch sensor
Plasmid#110696PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. fragilis 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6015nes
Plasmid#108657PurposeA S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-S6K-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI
Plasmid#115213PurposeExpresses RanCas13b from a SUMO-tagged bacterial expression constructDepositorInsertRanCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
JL002: pMVP/eGFP-DEST
Plasmid#121852PurposepMVP destination vector, empty expression vector backbone w/ eGFP selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6013x
Plasmid#108654PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertRaichu-EV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-PGK.Pro
Plasmid#79489PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoterDepositorInsertPGK Promoter (PGK1 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterPGKAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6017nes
Plasmid#108655PurposeA PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-PKA-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
JC001: pMVP/Ad/RGD-DEST
Plasmid#121844PurposepMVP destination vector, empty RGD fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-3G124-HilA
Plasmid#153323PurposeTriggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-3G124 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-AgdNK
Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
E. coli 16S toehold switch sensor
Plasmid#110698PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. coli 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CMV-GFP-BGHpA
Plasmid#190239PurposeExpresses EGFP in mammalian cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNA500
Plasmid#149583Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
JH401: pMVP/Ad/RGD-PK-DEST
Plasmid#121846PurposepMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only