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PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepInducer-20
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 15188
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlag-iCas9-P2A-GFP
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Insert Size (bp)5000
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Tag
/ Fusion Protein
- Flag-iCas9-P2A-GFP
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bypInducer20
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-iCas9-neo was a gift from Qin Yan (Addgene plasmid # 85400 ; http://n2t.net/addgene:85400 ; RRID:Addgene_85400) -
For your References section:
An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q. Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. 10.1093/nar/gkw660 PubMed 27458201