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Plasmid#186832PurposeExpresses FLAG-BMAL1-S515A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 515 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S516A
Plasmid#186833PurposeExpresses FLAG-BMAL1-S516A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 516 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S519A
Plasmid#186834PurposeExpresses FLAG-BMAL1-S519A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 519 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-S1pr1-R120A
Plasmid#203707PurposeOverexpression of FLAG-S1PR1-R120A mutant.DepositorInsertFlag-S1PR1-R120A (S1PR1 Human)
UseTagsFlagExpressionMammalianMutationR120APromoterAvailable sinceDec. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-3G124-HilA
Plasmid#153323PurposeTriggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-3G124 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (E1564A)
Plasmid#41587DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationE1564APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Orp1
Plasmid#65001PurposeExpression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-NLC-DHFR-SpCas9
Plasmid#124523PurposeExpresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cellsDepositorInsertNLC-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianMutationPromoterCbhAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCA528-AMOT(1-300)-sortase
Plasmid#166706Purposeexpresses AMOT(1-300) with C-terminal sortase tag in bacterial cellsDepositorInsertAMOT aa 1-300
UseTagsHis-SUMO and Sortase tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-PCAF (delta5-53a.a.)
Plasmid#65388PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertPCAF (KAT2B Human)
UseTagsEmGFP and V5ExpressionMammalianMutationlost 13-159bp of the ORFPromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc
Plasmid#86158PurposeFLS2 expressionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTags3xmycExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (D1320A)
Plasmid#41586DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationD1320APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL
Plasmid#107305PurposeExpresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cellsDepositorInsertSpCas9
UseCRISPRTags2x NLS and FKBPExpressionMammalianMutationR1335K and fused to FKBPPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-pac-T2A-iCre
Plasmid#113849PurposePuromycin resistance gene and iCre recombinaseDepositorInsertpac-T2A-iCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-PCP-eGFP
Plasmid#180274Purposeto epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertPCP-eGFP
UseTagseGFPExpressionInsectMutationPromoternanosAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-eSpCas9-D10A
Plasmid#80449Purposeenhanced SpCas9 mammalian expression vectorDepositorInsertehSpCas9_D10A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only