pCAGGS-Flag-S1pr1-R120A
(Plasmid
#203707)
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PurposeOverexpression of FLAG-S1PR1-R120A mutant.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlag-S1PR1-R120A
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Alt nameFlag-S1P1-R120A
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Alt nameEDG1-R120A
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SpeciesH. sapiens (human)
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MutationR120A
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Entrez GeneS1PR1 (a.k.a. CD363, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, S1P1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-Flag-S1pr1-R120A was a gift from Timothy Hla (Addgene plasmid # 203707 ; http://n2t.net/addgene:203707 ; RRID:Addgene_203707)