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Viral Prep#51502-AAV9PurposeReady-to-use AAV9 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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AAV pCAG-FLEX-EGFP-WPRE (AAV1)
Viral Prep#51502-AAV1PurposeReady-to-use AAV1 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV5)
Viral Prep#51502-AAV5PurposeReady-to-use AAV5 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV2)
Viral Prep#51502-AAV2PurposeReady-to-use AAV2 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV8)
Viral Prep#51502-AAV8PurposeReady-to-use AAV8 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV Retrograde)
Viral Prep#51502-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseTagsExpressionBacterialMutationPromoternative cas9 promoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#206994PurposePlasmid expressing both SpCas9 and SaCas9DepositorUseTagsP2AExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9_sgAAVS1
Plasmid#199213PurposeAll-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.ÂDepositorInsertAAVS1-gRNA
UseCRISPRTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-SB
Plasmid#169919PurposeExpression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-SB100X
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-SpCAS9-NG-pA
Plasmid#131465PurposeExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized SpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-N57
Plasmid#169920PurposeExpression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-N57
UseCRISPRTagsExpressionMutationN57 is the PAI domain of SB100XPromoterCAGAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LpCas12a-2AeGFP
Plasmid#123635PurposeMammalian expression, Genome editingDepositorInsertLpCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-2A-GFP-noITR
Plasmid#118415PurposeCas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expressionDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3
Plasmid#213973PurposeAAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locusDepositorInsertShank3 sgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb dPspCas13b + PspCas13b guide RNA
Plasmid#176306PurposeExpresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
UseTagsHA tagExpressionInsectMutationH133A, H1058APromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only